The cold storage time of kidney grafts needs to be identical
The cold storage time of kidney grafts needs to be identical
复制标题
肾移植物的冷藏时间需要相同
DOI：
10.1111/tri.12498
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发表时间：
2015-04
期刊：
影响因子：
--
通讯作者：
朱亮
中科院分区：
文献类型：
--
作者：
陈秋宇;朱亮
尊敬的先生们，我有一个关于 Anja Gallinat 等人的实验设计的问题。 （2014 年 6 月 30 日）[1]。在他们的研究中，猪肾脏在 4°C 的静态 HTK 中保存 18 小时。之后，他们将肾脏分为以下三组（分别为 n = 6）： 第 1 组：使用冷藏的肾脏进行实验，无需进一步处理，并作为对照。第 2 组：随后对冷藏肾脏进行低温修复，包括使用最近开发的含有 5 g% 葡聚糖 40 的 Custodiol-N 溶液，以 30 bpm 的脉动方式 (30/20 mmHg) 进行 1 小时的低温机器灌注。机器灌注期间不应用活性氧合。第3组：本组与第2组一样进行冷藏后低温复调，但机器灌注时间延长至4小时。我觉得这个实验组的设计并不严谨。在他们的实验中，三组的冷藏时间分别为18小时、19小时和22小时。在这样设计的前提下，我认为这些群体之间的对比并不严格。我建议实验组的设计如下：猪肾静态冷藏18 h（第1组）、静态冷藏17 h后低温机器灌注（第2组）、静态冷藏14 h后低温机器灌注4 h（第3组）。这样，各组的实验条件是一致的，可以进行比较。
Dear Sirs, I have a question for the experimental design of Anja Gallinat et al. (2014 Jun 30) [1]. In their research, porcine kidneys were preserved in static HTK at 4 °C for 18 h. After that they divided the kidneys into the following three groups (n = 6, respectively): Group 1: Cold-stored kidneys were used for the experiments without further treatment and served as controls. Group 2: Cold-stored kidneys were subsequently subjected to hypothermic reconditioning consisting of 1 h of hypothermic machine perfusion in a pulsatile manner (30/20 mmHg) at 30 bpm with the recently developed Custodiol-N solution containing 5 g % of dextran 40. No active oxygenation was applied during machine perfusion. Group 3: In this group, hypothermic reconditioning after cold storage was performed like in group 2, but the duration of machine perfusion was extended to 4 h. I don’t think this design of experimental groups is rigorous. In their experiment, cold storage time of the three groups is, respectively, 18 h, 19 h, and 22 h. Under the precondition of this design, I think the contrast between these groups is not strict. I suggest that the experimental groups need to be designed as below: porcine kidneys should be preserved by 18 h static cold storage (Group 1), 17 h static cold storage followed by 1 h hypothermic machine perfusion (Group 2), and 14 h static cold storage followed by 4 h hypothermic machine perfusion (Group 3).Thus, the experimental conditions of all the groups are consistent, they can be compared.
影响因子：
3.1
作者：
Gallinat, Anja;Efferz, Patrik;Minor, Thomas
通讯作者：
Minor, Thomas