Influence of intense sound exposure on glutathione synthesis in the cochlea
Influence of intense sound exposure on glutathione synthesis in the cochlea
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DOI：
10.1016/s0006-8993(98)00660-x
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发表时间：
1998-08
期刊：
影响因子：
2.9
通讯作者：
T. Yamasoba;C. Harris;Fumi Shoji;Rosanna J. Lee;A. Nuttall;Josef M. Miller
中科院分区：
文献类型：
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作者：
T. Yamasoba;C. Harris;Fumi Shoji;Rosanna J. Lee;A. Nuttall;Josef M. Miller
先前的研究已经表明，内源性谷胱甘肽（GSH）的消耗增强噪声诱导的听力损失（NIHL），而GSH的补充减弱NIHL（Yamasoba等人，784（1998）82-90）。由于这些研究结果表明，从NIHL的保护GSH的重要作用，我们评估了强的声音暴露（宽带噪声，105分贝SPL，5小时）的影响，GSH和半胱氨酸水平在豚鼠耳蜗使用高效液相色谱法。GSH水平在暴露后2和4 h显著升高，并在暴露后6 h恢复正常。感觉上皮和蜗轴中的GSH水平没有表现出显着的变化后噪声。半胱氨酸水平在任何耳蜗节段中均无变化。对于耳蜗作为一个整体，强烈的声音暴露没有显着改变GSH或半胱氨酸水平在整个6小时的测量期后曝光。这些结果表明，GSH合成显着上调选择性地在侧壁噪声暴露，大概是在响应于GSH的强大的消费，因为它是利用在清除活性氧。
Previous studies have shown that depletion of endogenous glutathione (GSH) potentiates noise-induced hearing loss (NIHL), whereas replenishment of GSH attenuates NIHL (Yamasoba et al., Brain Res. 784 (1998) 82–90). Since these findings indicate an important role of GSH in protection from NIHL, we assessed the influence of intense sound exposure (broadband noise, 105 dB SPL, 5 h) on GSH and cysteine levels in the guinea pig cochlea using high performance liquid chromatography. GSH levels were significantly increased in the lateral wall 2 and 4 h post-exposure and returned to normal 6 h post-exposure. GSH levels in the sensory epithelium and modiolus did not show significant changes following noise. Cysteine levels were unchanged in any of the cochlear segments. For the cochlea as a whole, intense sound exposure did not significantly change GSH or cysteine levels throughout the 6-h measurement period post-exposure. These results indicate that GSH synthesis is markedly upregulated selectively in the lateral wall by noise exposure, presumably in response to the robust consumption of GSH, as it is utilized in scavenging reactive oxygen species.