Raman and coherent anti-Stokes Raman scattering microspectroscopy for biomedical applications
Raman and coherent anti-Stokes Raman scattering microspectroscopy for biomedical applications
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DOI：
10.1117/1.jbo.17.4.040801
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发表时间：
2012-04-01
影响因子：
3.5
通讯作者：
Popp, Juergen
中科院分区：
文献类型：
--
作者：
Krafft, Christoph;Dietzek, Benjamin;Popp, Juergen
一篇教程文章介绍了在生物医学诊断中使用线性和非线性拉曼显微光谱。相干反斯托克斯拉曼散射（CARS）是拉曼光谱中应用最广泛的非线性变体。首先介绍了拉曼和CARS的基本概念，然后介绍了拉曼和CARS在生物医学上的应用。拉曼显微光谱学应用于体内和体外组织诊断，以及单个哺乳动物细胞的表征和鉴定。这些应用得益于拉曼光谱以无标记和无损的方式提供化学成分和分子结构的特定信息。将拉曼光谱的化学特异性与光学显微镜的空间分辨率相结合，可以记录具有分子对比度的高光谱图像。我们还详细介绍了拉曼光谱工具与其他技术的结合，如光镊、微流体和光纤探针。因此，我们旨在介绍现代生物光子学研究的一个令人兴奋的分支。(C) 2012年中国光学仪器工程师学会。(jbo.17.4.040801 DOI: 10.1117/1.)
A tutorial article is presented for the use of linear and nonlinear Raman microspectroscopies in biomedical diagnostics. Coherent anti-Stokes Raman scattering (CARS) is the most frequently applied nonlinear variant of Raman spectroscopy. The basic concepts of Raman and CARS are introduced first, and subsequent biomedical applications of Raman and CARS are described. Raman microspectroscopy is applied to both in-vivo and in-vitro tissue diagnostics, and the characterization and identification of individual mammalian cells. These applications benefit from the fact that Raman spectra provide specific information on the chemical composition and molecular structure in a label-free and nondestructive manner. Combining the chemical specificity of Raman spectroscopy with the spatial resolution of an optical microscope allows recording hyperspectral images with molecular contrast. We also elaborate on interfacing Raman spectroscopic tools with other technologies such as optical tweezing, microfluidics and fiber optic probes. Thereby, we aim at presenting a guide into one exciting branch of modern biophotonics research. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.4.040801]