NG2-expressing glial progenitor cells: an abundant and widespread population of cycling cells in the adult rat CNS
NG2-expressing glial progenitor cells: an abundant and widespread population of cycling cells in the adult rat CNS
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DOI：
10.1016/s1044-7431(03)00210-0
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发表时间：
2003-10-01
影响因子：
3.5
通讯作者：
Reynolds, R
中科院分区：
文献类型：
--
作者：
Dawson, MRL;Polito, A;Reynolds, R
发育中的中枢神经系统的神经胶质前体细胞致力于少突胶质细胞系(OPC)表达硫酸软骨素蛋白多糖NG2。部分OPC未能分化过NG2和脂类抗原O4的表达阶段，并以表型不成熟的形式存在于成年中枢神经系统中。然而，NG2(+)细胞在成人中枢神经系统中的生理功能尚不清楚。使用针对NG2的抗体，我们发现NG2在成熟的中枢神经系统中由不同的细胞群表达，具有少突胶质前体细胞的均一抗原表型。NG2(+)OPC的形态因地区而异，反映了不同的结构环境，但它们似乎代表了任何一个灰质或白质区域内的同质群体。一项对九个中枢神经系统区域的研究表明，NG2(+)OPC在整个中枢神经系统中数量众多，白质中的数量仅为灰色区域的1.5倍。脊髓灰质和白质中OPC与髓鞘少突胶质细胞的比例约为1：4，而前脑灰质区域的比例为1：1，这一现象将导致脱髓鞘后少突胶质细胞的替换。BrdU掺入实验表明，NG2(+)细胞是成年大鼠中枢神经系统的主要分裂细胞群。由于在脉冲追逐10天后，少突胶质细胞中几乎没有检测到凋亡，BrdU的存在越来越多，伴随着NG2(+)BrdU结合细胞的减少，我们认为在成年FIFE中，少突胶质细胞群体的大小实际上可能会增加。(C)2003 Elsevier Inc.保留所有权利。
Glial progenitor cells of the developing CNS committed to the oligodendrocyte lineage (OPCs) express the chondroitin sulfate proteoglycan, NG2. A proportion of OPCs fail to differentiate past the stage at which they express NG2 and the lipid antigen O4 and persist in the adult CNS in a phenotypically immature form. However, the physiological function of NG2(+) cells in the adult CNS is unknown. Using antibodies against NG2 we show that NG2 is expressed by a distinct cell population in the mature CNS with the homogeneous antigenic phenotype of oligodendrocyte progenitors. The morphology of NG2(+) OPCs varies from region to region, reflecting the different structural environments, but they appear to represent a homogeneous population within any one gray or white matter region. A study of nine CNS regions showed that NG2(+) OPCs are numerous throughout the CNS and numbers in the white matter are only 1.5 times that in the gray. Whereas the ratio of OPCs to myelinating oligodendrocytes in the spinal cord gray and white matter approximates 1:4, gray matter regions of the forebrain have a 1:1 ratio, a phenomenon that will have consequences for oligodendrocyte replacement following demyelination. BrdU incorporation experiments showed that NG2(+) cells are the major dividing cell population of the adult rat CNS. Since very little apoptosis was detected and BrdU became increasingly present in oligodendrocytes after a 10-day pulse chase, with a concomitant decrease in NG2(+) BrdU incorporating cells, we suggest that the size of the oligodendrocyte population may actually increase during adult fife. (C) 2003 Elsevier Inc. All rights reserved.