Protein phosphorylation mediated by partially purified avian sarcoma virus transforming-gene product.
Protein phosphorylation mediated by partially purified avian sarcoma virus transforming-gene product.
复制标题
由部分纯化的禽肉瘤病毒转化基因产物介导的蛋白质磷酸化。
DOI：
10.1101/sqb.1980.044.01.098
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Brugge,JS
中科院分区：
文献类型：
--
作者：
Erikson,RI;Collett,MS;Erikson,E;Purchio,AF;Brugge,JS
材料和方法细胞提取物的制备和免疫印迹。这些实验中使用的细胞是由ASV A亚群的Schmidt-Ruppin（SR）株或由该株的温度敏感转化突变体NY 68转化的鸡胚成纤维细胞（Kawai和Hanafusa 1971）;欧洲田鼠SR ASV转化的（田鼠）细胞（克隆lT）（由明尼苏达大学医学院微生物学系AJ Faras提供）;和在本实验室培养物中建立的SR-ASV转化的小鼠细胞（Balb/c），其来自通过注射5x 106个SR-ASV转化的细胞在小鼠中诱导的肿瘤（最初获自JT Parsons，Department of Microbiology，University of Virginia Medical School）。荷瘤兔（TBR）血清获自New
MATERIALS AND METHODSPreparation of cell extracts and immunopredpltation. The cells used in these experiments were chick embryo fibroblasts transformed by the Schmidt-Ruppin (SR) strain of ASV, subgroup A, or by the temperature-sensitive transformation mutant of this strain, NY68 (Kawai and Hanafusa 1971); European field vole (Microtus agrestis) cells transformed by SR ASV (clone lT)(provided by AJ Faras, Department of Microbiology, University of Minnesota Medical School); and SR-ASV-transformed mouse cells (Balb/c) established in culture in this laboratory from tumors induced in mice by the injection of 5x106 SR-ASV-transformed cells (originally obtained from JT Parsons, Department of Microbiology, University of Virginia Medical School). Tumorbearing rabbit (TBR) serum was obtained from New