Quantification of phosphocholine and glycerophosphocholine with 31P edited 1H NMR spectroscopy
Quantification of phosphocholine and glycerophosphocholine with 31P edited 1H NMR spectroscopy
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DOI：
10.1002/nbm.973
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发表时间：
2005-11-01
影响因子：
2.9
通讯作者：
Cheng, LL
中科院分区：
文献类型：
--
作者：
Loening, NM;Chamberlin, AM;Cheng, LL
胆碱及其相关化合物磷酸胆碱（PC）和甘油磷酸胆碱（GPC）被认为是肿瘤学中重要的代谢物。过去的研究已经证明了这些代谢物的相对比例和浓度与癌症的发生和进展之间的相关性。目前，体内和组织离体磁共振波谱法多集中于测量这些代谢物的总浓度，难以区分。本文报道了一种使用P-31编辑H-1光谱来定量生物样品中胆碱、PC和GPC浓度的新方案，并使用人类脑肿瘤提取物样品证明了其适用性。这种方法特别适合于PC和GPC共振不能充分分辨和/或被其他代谢物遮蔽的分析情况。因此，该方案有可能用于体外组织样品中胆碱化合物的分析。版权所有(c) 2005 John Wiley & Sons, Ltd。
Choline and the related compounds phosphocholine (PC) and glycerophosphocholine (GPC) are considered to be important metabolites in oncology. Past studies have demonstrated correlations linking the relative ratios and concentrations of these metabolites with the development and progression of cancer. Currently, in vivo and tissue ex vivo magnetic resonance spectroscopy methods have mostly centered on measuring the total concentration of these metabolites and have difficulty in differentiating between them. Here, a new scheme that uses P-31 edited H-1 spectroscopy to quantify the concentrations of choline, PC and GPC in biological samples is reported and its applicability is demonstrated using samples of human brain tumor extracts. This method is particularly well-suited for analytical situations where the PC and GPC resonances are not sufficiently resolved and/or are obscured by other metabolites. Consequently, this scheme has the potential to be used for the analysis of choline compounds in ex vivo tissue samples. Copyright (c) 2005 John Wiley & Sons, Ltd.