Morphology change and detachment of lipid bilayers from the mica substrate driven by graphene oxide sheets.
Morphology change and detachment of lipid bilayers from the mica substrate driven by graphene oxide sheets.
复制标题
DOI：
10.1021/la500788z
复制
发表时间：
2014-04
期刊：
影响因子：
--
通讯作者：
Haozhi Lei;Xuejiao Zhou;Haixia Wu;Yang Song;Jun Hu;Shouwu Guo;Yi Zhang
中科院分区：
文献类型：
--
作者：
Haozhi Lei;Xuejiao Zhou;Haixia Wu;Yang Song;Jun Hu;Shouwu Guo;Yi Zhang
了解氧化石墨烯与脂膜之间的相互作用对于探索氧化石墨烯的生物相容性和细胞毒性具有重要意义，这是氧化石墨烯在生物传感器、生物成像、药物传递、抗菌等领域应用的基础。在本文中，我们利用原位原子力显微镜（AFM）成像技术监测了氧化石墨烯薄片对云母基质上脂质双分子层形态变化和脱离的动态过程。研究发现，在氧化石墨烯的诱导下，裸脂双分子层高度急剧膨胀，且不稳定，易与云母基质分离。分离的脂质分子被发现与氧化石墨烯表面结合。结果还表明，氧化石墨烯可能通过吸附用于稳定云母基质上双层结构的金属离子（如钙离子）来影响支撑脂质双层结构（slb）的高度和稳定性。这些发现说明了氧化石墨烯对slb的复杂影响，应该有助于未来氧化石墨烯在生物技术中的应用。
Understanding the interaction between graphene oxide (GO) and a lipid membrane is significant for exploring the biocompatibility and cytotoxicity of GO, which is the basis for utilizing GO in the fields of biosensors, bioimaging, drug delivery, antibacterials, and so on. In this article, we monitored the dynamic process of the morphology change and detachment of lipid bilayers on mica substrates prompted by GO sheets by in situ atomic force microscope (AFM) imaging. It was found that the bare lipid bilayer dramatically expanded in height and would be unstable and detachable from the mica substrates as induced by GO. The detached lipid molecules were found to bind to the GO surface. The results also imply that GO is likely to influence the height and stability of the supported lipid bilayers (SLBs) by adsorbing metal ions such as calcium ions that were used to stabilize the bilayer structures on the mica substrate. These findings illustrate a complicated effect of GO on the SLBs and should be helpful in future applications of GO in biotechnology.