The vaccinia virus A27L protein is needed for the microtubule-dependent transport of intracellular mature virus particles
The vaccinia virus A27L protein is needed for the microtubule-dependent transport of intracellular mature virus particles
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DOI：
10.1099/0022-1317-81-1-47
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发表时间：
2000-01-01
影响因子：
3.8
通讯作者：
Smith, GL
中科院分区：
文献类型：
--
作者：
Sanderson, CM;Hollinshead, M;Smith, GL
牛痘病毒（VV）A27 L基因编码一种14 kDa的蛋白质，该蛋白质是形成细胞内包膜病毒（IEV）以及因此形成正常大小噬斑所必需的。此处提供的数据表明，A27 L在验证与确认组装中发挥额外作用。当用VV WR 32 -7/Ind 1 4K感染细胞时，在抑制A27 L表达的条件下，来自病毒工厂的细胞内成熟病毒（IMV）的转运被抑制，并且发现一些IMV与病毒新月体异常相关。相反，在IEV形成中有缺陷的其他VV突变体（V Delta B5 R和V Delta F13 L）产生被运输出病毒工厂的IMV颗粒。这表明A27 L在IMV运输中的特定作用。诱导A27 L表达在10小时后感染促进分散的IMV颗粒集群，但只有当微管是完整的。当细胞感染WR 32 -7/14 K（一种表达A27 L蛋白突变形式的VV毒株）时，IEV颗粒的形成也受损;然而，这种突变并不抑制IMV颗粒的细胞内转运。总之，这些数据定义了VV形态发生的两个新方面，首先，A27 L是IMV运输和导致IEV形成的消化过程所必需的，其次，IMV颗粒在病毒工厂和IEV形成位点之间的运动是微管依赖性的。
The vaccinia virus (VV) A27L gene encodes a 14 kDa protein that is required for the formation of intracellular enveloped virus (IEV) and, consequently, normal sized plaques. Data presented here show that A27L plays an additional role in VV assembly. When cells were infected with the VV WR32-7/Ind 1 4K, under conditions that repress A27L expression, transport of intracellular mature virus (IMV) from virus factories was inhibited and some IMV was found in aberrant association with virus crescents. In contrast, other VV mutants (v Delta B5R and V Delta F13L) that are defective in IEV formation produce IMV particles that are transported out of virus factories. This indicated a specific role for A27L in IMV transport. Induction of A27L expression at 10 h post-infection promoted the dispersal of clustered IMV particles, but only when microtubules were intact. Formation of IEV particles was also impaired when cells were infected with WR32-7/14K, a VV strain expressing a mutated form of the A27 L protein; however, this mutation did not inhibit intracellular transport of IMV particles. Collectively, these data define two novel aspects of VV morphogenesis, Firstly, A27L is required for both IMV transport and the process of envelopment that leads to IEV formation, Secondly, movement of IMV particles between the virus factory and the site of IEV formation is microtubule-dependent.