Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by Ralstonia eutropha
Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by Ralstonia eutropha
复制标题
DOI：
10.1016/j.bej.2005.01.016
复制
发表时间：
2005-04-01
影响因子：
3.9
通讯作者：
Kim, BS
中科院分区：
文献类型：
--
作者：
Kim, JS;Lee, BH;Kim, BS
生长真养罗尔斯通氏菌ATCC 17699以从果糖和γ-丁内酯产生聚（3-羟基丁酸酯-co-4-羟基丁酸酯）[P（3 HB-co-4 HB）]。通过摇瓶培养，发现R.高浓度的果糖（大于20 g/L）和γ-丁内酯（大于6 g/L）对真养型细胞有抑制作用。通过添加少量（2g/L）乙酸盐或丙酸盐作为刺激剂，4 HB分数可以从37.9mol%增加到53.7mol%。进行分批补料培养（DO-stat和恒定补料）以提高细胞和PHA浓度。在第一步中，通过DO-stat补料果糖使细胞生长至38-48 g/L。应用氮限制，并将果糖进料用果糖和γ-丁内酯的混合物代替。考察了第二步料液中γ-丁内酯与果糖比例的影响。随着比例的增加，4 HB分数增加，而细胞和PHA浓度，PHA含量和生产力下降。得到的结果在33.6-49.1 g/L的细胞浓度、13.8-24.4 g/L的PHA浓度、38.8-50.2%的PHA含量、1.64-25.2 mol%的4 HB级分和0.32-0.55 g/L/h的生产率的范围内。(C)2004 Elsevier B. V.保留所有权利。
Ralstonia eutropha ATCC 17699 was grown to produce poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] from fructose and gamma-butyrolactone. Through flask cultures, it was found that R. eutropha cells were inhibited by high concentrations of fructose (more than 20 g/L) and gamma-butyrolactone (more than 6 g/L). The 4HB fraction could be increased from 37.9 to 53.7 mol% by adding small amount (2 g/L) of acetate or propionate as a stimulator. Fed-batch cultures (DO-stat and constant feeding) were carried out to improve cell and PHA concentration. In the first step, cells were grown to 38-48 g/L by DO-stat feeding of fructose. Nitrogen limitation was applied and the fructose feed was replaced with the mixture of fructose and gamma-butyrolactone. The effects of gamma-butyrolactone to fructose ratio in the second step feeding solution were examined. As the ratio increased, 4HB fraction increased, while cell and PHA concentration, PHA content, and productivity decreased. The results obtained were in the range of 33.6-49.1 g/L of cell concentration, 13.8-24.4 g/L of PHA concentration, 38.8-50.2% of PHA content, 1.64-25.2 mol% of 4HB fraction, and 0.32-0.55 g/L/h of productivity. (C) 2004 Elsevier B.V. All rights reserved.