Evidence for an interaction between canine synovial cell proteoglycans and link proteins.
Evidence for an interaction between canine synovial cell proteoglycans and link proteins.
复制标题
犬滑膜细胞蛋白聚糖和连接蛋白之间相互作用的证据。
DOI：
10.1016/0304-4165(85)90144-8
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Myers,SL
中科院分区：
文献类型：
--
作者：
Fife,RS;Myers,SL
连接蛋白是使软骨中的蛋白聚糖和透明质酸的聚集体稳定的糖蛋白。我们最近在犬滑膜细胞培养物中鉴定了连接蛋白。我们现在发现，连接蛋白和蛋白聚糖从这些细胞中提取的解离条件下沉淀在高浮力密度的相关氯化铯密度梯度的馏分，这表明连接蛋白与高浮力密度蛋白聚糖相互作用。在含有[35S]硫酸盐标记的滑膜细胞提取物的梯度中，在高浮力密度组分中发现76%的标记硫酸盐和54%的糖醛酸。在缔合条件下，粗滑膜细胞提取物、滑膜细胞高浮力密度级分和培养基的Sepharose 2B洗脱曲线表明滑膜细胞蛋白聚糖以单体形式存在，而不是聚集体。在相同条件下，滑膜细胞连接蛋白与[35S]硫酸盐标记的材料共沉淀。这些蛋白聚糖在体外不与外源性透明质酸相互作用。硫酸皮肤素、硫酸软骨素和硫酸乙酰肝素是由培养的犬滑膜细胞合成的主要细胞相关硫酸化糖胺聚糖，而在培养基中发现了透明质酸。虽然由培养的滑膜细胞合成的蛋白聚糖与连接蛋白相互作用，但这些数据表明它们不与透明质酸相互作用以形成聚集体。
Link proteins are glycoproteins which stabilize aggregates of proteoglycans and hyaluronic acid in cartilage. We recently identified link proteins in canine synovial cell cultures. We now find that link proteins and proteoglycans extracted from these cells under dissociative conditions sediment in the high-buoyant-density fractions of an associative cesium chloride density gradient, suggesting that link proteins interact with high-bouyant-density proteoglycans. In gradients containing [35S]sulfate-labeled synovial cell extracts, 76% of the labeled sulfate and 54% of the uronic acid is found in the high-buoyant-density fractions. Under associative conditions, Sepharose 2B elution profiles of the crude synovial cell extract, synovial cell high-buoyant-density fractions, and culture medium indicate that synovial cell proteoglycans are present in monomeric form, rather than in aggregates. Synovial cell link proteins co-elute with the [35S]sulfate-labeled material under the same conditions. These proteoglycans do not interact in vitro with exogenous hyaluronic acid. Dermatan sulfate, chondroitin sulfate and heparan sulfate are the major cell-associated sulfated glycosaminoglycans synthesized by cultured canine synovial cells, while hyaluronic acid is found in the culture medium. Although the proteoglycans synthesized by cultured synovial cells interact with link proteins, these data indicate that they do not interact with hyaluronic acid to form aggregates.