Application of Fenton reaction for nanomolar determination of hydrogen peroxide in seawater.
Application of Fenton reaction for nanomolar determination of hydrogen peroxide in seawater.
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DOI：
10.1016/j.aca.2008.08.026
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发表时间：
2008-10
影响因子：
6.2
通讯作者：
E. Olasehinde;S. Makino;H. Kondo;K. Takeda;H. Sakugawa
中科院分区：
文献类型：
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作者：
E. Olasehinde;S. Makino;H. Kondo;K. Takeda;H. Sakugawa
一种用于测定海水中纳摩尔水平过氧化氢 (H2O2) 的简单而灵敏的方法已经开发出来并得到验证。该方法基于亚铁在酸性溶液中还原H2O2产生羟基自由基(OH)，该羟基自由基与苯反应生成苯酚。通过反相高效液相色谱从反应混合物中分离苯酚，并分别在270nm和298nm的激发和发射处测量其荧光强度信号。在最佳条件下，校准曲线在(0~50)×103nmolL−1H2O2范围内呈线性。 500 和 50nmolL−1H2O2 的五次重复测量的相对标准偏差分别为 1.9 和 2.4%。 H2O2 的检测限定义为海水中最低标准溶液 (5nmolL−1H2O2) 标准偏差的三倍，为 4nmolL−1。还研究了亚硝酸根离子（NO2−）对苯酚荧光强度的干扰。结果表明，在海水样品中添加10μmolL−1NO2−没有表现出明显的干扰，但在海水样品中添加50μmolL−1NO2−使苯酚的荧光强度信号降低了近40%。该方法与广为接受的（对羟基苯基）乙酸（POHPAA）-FIA 方法的相互比较显示出极好的一致性。该方法已应用于濑户内陆海水样品中 H2O2 的船上分析。
A simple and sensitive method for the determination of nanomolar levels of hydrogen peroxide (H2O2) in seawater has been developed and validated. This method is based on the reduction of H2O2by ferrous iron in acid solution to yield hydroxyl radical (OH) which reacts with benzene to produce phenol. Phenol is separated from the reaction mixture by reversed phase high performance liquid chromatography and its fluorescence intensity signals were measured at excitation and emission of 270 and 298nm, respectively. Under optimum conditions, the calibration curve exhibited linearity in the range of (0–50)×103nmolL−1H2O2. The relative standard deviations for five replicate measurements of 500 and 50nmolL−1H2O2are 1.9 and 2.4%, respectively. The detection limit for H2O2, defined as three times the standard deviation of the lowest standard solution (5nmolL−1H2O2) in seawater is 4nmolL−1. Interference of nitrite ion (NO2−) on the fluorescence intensity of phenol was also investigated. The result indicated that the addition of 10μmolL−1NO2−to seawater samples showed no significant interference, although, the addition of 50μmolL−1NO2−to the seawater samples decreases the fluorescence intensity signals of phenol by almost 40%. Intercomparison of this method with well-accepted (p-hydroxyphenyl) acetic acid (POHPAA)-FIA method shows excellent agreement. The proposed method has been applied on-board analysis of H2O2in Seto Inland seawater samples.