Confirmation of the structure of lipid A derived from the lipopolysaccharide of Rhodobacter sphaeroides by a combination of MALDI, LSIMS, and tandem mass spectrometry.
Confirmation of the structure of lipid A derived from the lipopolysaccharide of Rhodobacter sphaeroides by a combination of MALDI, LSIMS, and tandem mass spectrometry.
复制标题
通过 MALDI、LSIMS 和串联质谱法的组合确认源自球形红杆菌脂多糖的脂质 A 的结构。
DOI：
10.1021/ac9612943
复制
发表时间：
1997
影响因子：
7.4
通讯作者：
Qureshi,N
中科院分区：
文献类型：
--
作者：
Kaltashov,IA;Doroshenko,V;Cotter,RJ;Takayama,K;Qureshi,N
使用 LSIMS（在双扇区质谱仪上）和 MALDI（在飞行时间和离子阱质谱仪上）以及正负离子模式下的串联质谱仪相结合，确认了来自球形红细菌的无毒二磷酰脂质 A 的化学结构。精确的分子量测量以及碎片离子质量的分析得出了脂肪酰基的组成。还进行了串联实验（碰撞诱导准分子和氧鎓离子的解离），揭示了二糖主链上脂肪酰基的精确位置和性质。
The chemical structure of nontoxic diphosphoryl lipid A fromRhodobacter sphaeroideswas confirmed using a combination of LSIMS (on a two-sector mass spectrometer) and MALDI (on time-of-flight and ion trap mass spectrometers) in conjunction with tandem mass spectrometry in both positive and negative ion modes. Accurate molecular weight measurement accompanied by the analysis of fragment ion masses yielded the composition of fatty acyl groups. Tandem experiments (collisionally induced dissociation of both quasimolecular and oxonium ions) were also performed, revealing the precise location and nature of the fatty acyl groups on the disaccharide backbone.