Rapid preparation of Thermus flavus DNA polymerase.
Rapid preparation of Thermus flavus DNA polymerase.
复制标题
黄栖热菌 DNA 聚合酶的快速制备。
DOI：
10.1101/gr.3.6.372
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Hart,RP
中科院分区：
文献类型：
--
作者：
Harrell2nd,RA;Hart,RP
Thermus thermophilus（3'4）和Thermus caldophilus。(s)我们发现最适合我们需要的聚合酶（6，7）是从嗜热菌T. flavus（例如，HotTub、阿默舍姆或Pyrostase、分子遗传资源）。霸王先前已经分离并表征了黄DNA聚合酶。（8'9）不幸的是，公开的DNA聚合酶分离方法费力且效率低。因此，我们开发了一种适合我们需要的快速制备方法。图1给出了概述我们的过程的流程图。
Thermus thermophilus (3'4) and Thermus caldophilus.(s) The polymerase that we found to be most suitable for our needs (6, 7) was isolated from the thermophilic bacteria T. flavus (eg, HotTub, Amersham, or Pyrostase, Molecular Genetics Resources). A T. flavus DNA polymerase has been isolated and characterized previously.(8'9) Unfortunately, the published procedure for DNA polymerase isolation was laborious and inefficient. Therefore, we developed a rapid preparation method suited to our needs. A flow diagram outlining our procedure is given in Figure 1.