Quantification of jasmonic and salicylic acids in rice seedling leaves.
Quantification of jasmonic and salicylic acids in rice seedling leaves.
复制标题
DOI：
10.1007/978-1-62703-194-3_13
复制
发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Rakwal, Randeep
中科院分区：
文献类型：
--
作者：
Cho, Kyoungwon;Han, Oksoo;Rakwal, Randeep
茉莉酸 (JA) 和水杨酸 (SA) 是参与植物生长、发育和防御各个方面的关键信号成分。它们的组成水平因植物而异，并且同一植物内的组织也因组织而异。此外，当植物暴露于生物和非生物胁迫时，它们的数量水平会发生变化。为了更好地了解 JA 和 SA 介导的信号传导和代谢途径，精确量化它们在植物/组织/器官中的水平非常重要。然而，它们的提取和定量并非微不足道，并且在技术上仍然具有挑战性。各个实验室都在努力开发一种简单且标准的程序，可用于 JA 和 SA 的定量。在这里，我们介绍了实验程序以及我们十年来在稻苗叶子中以绝对方式提取和定量它们的经验。我们必须提及的是，该方法已应用于单子叶植物和双子叶植物，以对 JA 和 SA 进行绝对定量。由于合作是科学技术快速进步的关键，我们始终愿意与任何活跃的研究小组分享我们在该领域的经验，旨在进一步改进程序，并最终将它们（JA 和 SA）定量水平的重要性与植物中的信号和代谢途径网络联系起来。
Jasmonic acid (JA) and salicylic acid (SA) are critical signaling components involved in various aspects of plant growth, development, and defense. Their constitutive levels vary from plant to plant and also from tissue to tissue within the same plant. Moreover, their quantitative levels change when plant is exposed to biotic and abiotic stresses. To better understand the JA- and SA-mediated signaling and metabolic pathways, it is important to precisely quantify their levels in plants/tissues/organs. However, their extraction and quantification are not trivial and still technically challenging. An effort has been made in various laboratories to develop a simple and standard procedure that can be utilized for quantification of JA and SA. Here, we present the experimental procedure and our decade of experience on extracting and quantifying them in an absolute manner in leaves of rice seedlings. We must mention that this method has been applied to both monocotyledonous and dicotyledonous plants for absolute quantification of JA and SA. As collaboration is the key towards rapid progress in science and technology, we are always open to sharing our experience in this field with any active research group with an aim to improve the procedure further and eventually to connect the importance of their (JA and SA) quantitative levels with networks of signaling and metabolic pathways in plants.