Unexpected role of the IMD pathway in Drosophila gut defense against Staphylococcus aureus
Unexpected role of the IMD pathway in Drosophila gut defense against Staphylococcus aureus
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DOI：
10.1016/j.bbrc.2017.11.004
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发表时间：
2018-01-01
影响因子：
3.1
通讯作者：
Kuraishi, Takayuki
中科院分区：
文献类型：
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作者：
Hori, Aki;Kurata, Shoichiro;Kuraishi, Takayuki
在本研究中，果蝇属果蝇被用作研究天然免疫的分子机制的合适的模式动物。为了对抗经口传播的致病性革兰氏阴性菌，果蝇肠道配备了围食基质，这是一种由几丁质和糖蛋白组成的物理屏障：Duox系统产生活性氧（ROS），进而杀灭感染的微生物，IMD途径调节抗菌肽（AMP）的表达，进而控制ROS抗性病原体。然而，很少有人知道对革兰氏阳性菌在苍蝇肠道的防御机制。在这里，我们表明围食基质保护果蝇免受革兰氏阳性菌S。金黄色。我们还定义了几个角色的活性氧在响应感染，并表明IMD途径所需的清除摄入的微生物，可能独立于AMP的表达。这些发现为果蝇肠道防御系统的研究提供了一个新的视角，有助于阐明肠道微生物的共生过程和致病机制。(C)2017作者爱思唯尔公司出版
In this study, fruit fly of the genus Drosophila is utilized as a suitable model animal to investigate the molecular mechanisms of innate immunity. To combat orally transmitted pathogenic Gram-negative bacteria, the Drosophila gut is armed with the peritrophic matrix, which is a physical barrier composed of chitin and glycoproteins: the Duox system that produces reactive oxygen species (ROS), which in turn sterilize infected microbes, and the IMD pathway that regulates the expression of antimicrobial peptides (AMPs), which in turn control ROS-resistant pathogens. However, little is known about the defense mechanisms against Gram-positive bacteria in the fly gut. Here, we show that the peritrophic matrix protects Drosophila against Gram-positive bacteria S. aureus. We also define the few roles of ROS in response to the infection and show that the IMD pathway is required for the clearance of ingested microbes, possibly independently from AMP expression. These findings provide a new aspect of the gut defense system of Drosophila, and helps to elucidate the processes of gut-microbe symbiosis and pathogenesis. (C) 2017 The Authors. Published by Elsevier Inc.