Transgenic expression of dual positional maize lipoxygenase-1 leads to the regulation of defense-related signaling molecules and activation of the antioxidative enzyme system in rice
Transgenic expression of dual positional maize lipoxygenase-1 leads to the regulation of defense-related signaling molecules and activation of the antioxidative enzyme system in rice
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DOI：
10.1016/j.plantsci.2011.10.016
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发表时间：
2012-04-01
期刊：
影响因子：
5.2
通讯作者：
Han, Oksoo
中科院分区：
文献类型：
--
作者：
Cho, Kyoungwon;Kim, Young Cheol;Han, Oksoo
分析了双位置玉米脂氧化酶-1转基因表达对水稻防御系统的影响。氢过氧化物酶和抗氧化酶（超氧化物歧化酶、过氧化氢酶、过氧化物酶）的活性增加，醛类（包括丙二醛）含量高。茉莉素的组成水平略有升高，水杨酸的组成水平略有下降。损伤反应动力学分析表明，茉莉酸和水杨酸在纯转基因水稻植株中呈负相关，表明茉莉酸和水杨酸之间存在拮抗相互作用。基因芯片分析表明，转基因水稻中编码抗氧化酶和病原菌相关蛋白的防御相关基因上调，对稻瘟病菌的抗性增强。综上所述，我们的研究结果表明，在细胞质中表达的玉米脂氧合酶-1对转基因水稻包括抗氧化酶在内的防御系统起着重要的调节作用，这些作用可能是通过玉米脂氧合酶-1酶催化过氧化机制产生的活性氧介导的。2011爱思唯尔爱尔兰有限公司版权所有。
Effects of transgenic expression of dual positional maize lipoxygenase-1 on the defense system were analyzed in rice. The activities of hydroperoxidelyase and antioxidative enzymes (superoxide dismutase, catalase, peroxidase) were increased and high levels of aldehydes including malondialdehyde were produced. The constitutive level of jasmonic was slightly increased and the constitutive salicylic acid level was decreased. Kinetic analysis of wound response indicated that the levels of jasmonic acid and salicylic acid are inversely correlated in nully transgenic rice plants, suggesting that there is an antagonistic interaction between jasmonic acid and salicylic acid. Microarray analysis indicated that several defense-related genes encoding antioxidative enzymes and pathogen-related proteins were up-regulated, and the resistance to rice blast fungus was enhanced in transgenic rice. Taken together, our results suggest that maize lipoxygenase-1 expressed in the cytoplasm plays an important role for the regulation of defense system including the antioxidative enzymes in transgenic rice, and that these effects may be mediated by reactive oxygen species generated through the enzyme-initiated catalytic peroxidation mechanism of maize lipoxygenase-1. (c) 2011 Elsevier Ireland Ltd. All rights reserved.