Glacial refugia of limber pine (Pinus flexilis James) inferred from the population structure of mitochondrial DNA
Glacial refugia of limber pine (Pinus flexilis James) inferred from the population structure of mitochondrial DNA
复制标题
从线粒体 DNA 种群结构推断软松 (Pinus flexilis James) 冰川避难所
作者：
J. Mitton;BR Kreiser;Robert G. Latta
为了推断软木松栖息的冰川避难所，我们研究了线粒体DNA(MtDNA)在NaD1第二内含子上的8个大小变异的全范围种群结构。数据包括来自40个地点的704棵树的单倍型。这些种群的Fst值为0.80，远高于已报道的樟子松等位酶和叶绿体DNA(CpDNA)的值，这表明每代在不同地点之间移动的种子数量为≈0.12。如此大规模的基因流将允许突变和随后的遗传漂移对线粒体DNA的种群结构产生重大影响。大多数mtDNA单倍型局限于地理范围的较小部分。这些数据与七个冰川避难所的mtDNA分化一致，随后又散布出这些避难所。
To make inferences about the glacial refugia that harboured the limber pine, Pinus flexilis James, we examined the range‐wide population structure of mitochondrial DNA (mtDNA) with eight size variants in the second intron of nad1. The data consisted of haplotypes from 704 trees collected from 40 localities. The value of FST for these populations was 0.80, which is a much larger value than has been reported for allozymes and chloroplast DNA (cpDNA) in limber pine, and it suggests that the number of seeds moving among localities per generation is ≈ 0.12. Gene flow of this magnitude would allow mutation and subsequent genetic drift to have a substantial impact on the population structure of mtDNA. The majority of the mtDNA haplotypes are restricted to minor portions of the geographical range. The data are consistent with mtDNA differentiation in seven glacial refugia, followed by dispersal out of those refugia.