The Putative Methyltransferase Tl LAE1 Is Involved in the Regulation of Peptaibols Production in the Biocontrol Fungus Trichoderma longibrachiatum SMF2
The Putative Methyltransferase Tl LAE1 Is Involved in the Regulation of Peptaibols Production in the Biocontrol Fungus Trichoderma longibrachiatum SMF2
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DOI：
10.3389/fmicb.2020.01267
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发表时间：
2020-06-12
影响因子：
5.2
通讯作者：
Song, Xiao-Yan
中科院分区：
文献类型：
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作者：
Shi, Jin-Chao;Shi, Wei-Ling;Song, Xiao-Yan
生防真菌长枝木霉（Trichoderma longibrachiatum）SMF 2分泌的大量肽类物质具有广泛的生物活性，具有很大的应用价值。然而，其调控表达的机制尚不清楚。甲基转移酶LaeA/LAE 1是丝状真菌中一个参与次生代谢产物生物合成的全局调节因子。在这项研究中，我们证明了LaeA/LAE 1在生防真菌T. longibrachriatum SMF 2，TlLAE 1，在调节肽酶产生中起重要作用。Tllae 1基因的缺失导致菌丝生长受到轻微的负面影响，并且分生孢子产量显著下降。Tllae 1的缺失也在很大程度上损害了肽酶的产生。进一步的分析表明，这种缺陷发生在两个合成酶编码基因tlx 1和tlx 2的转录水平上，这两个合成酶编码基因负责肽酶的产生。Tllae 1在T. longibrachiatumSMF 2导致肽酶产量增加2倍，表明这是提高木霉属真菌中肽酶产量的策略。这些结果证明了LAE 1在调节长臂嗜热链球菌SMF 2中的肽酶产生中的重要作用。
The biocontrol fungusTrichoderma longibrachiatumSMF2 secretes a large quantity of peptaibols that have been shown to have a range of biological activities and therefore great application values. However, the mechanism of the regulatory expression of peptaibols is still unclear. The putative methyltransferase LaeA/LAE1 is a global regulator involved in the biosynthesis of some secondary metabolites in filamentous fungi. In this study, we demonstrated that the ortholog of LaeA/LAE1 in the biocontrol fungusT. longibrachiatumSMF2,TlLAE1, plays an important role in the regulation of peptaibols production. Deletion ofTllae1resulted in a slight negative impact on mycelial growth, and a significant defect in conidial production. Deletion ofTllae1also compromised the production of peptaibols to a large degree. Further analyses indicated that this defect occurred at the transcriptional level of the two synthetases-encoding genes,tlx1andtlx2, which are responsible for peptaibols production. By contrast, constitutive expression ofTllae1inT. longibrachiatumSMF2 led to 2-fold increased peptaibols production, suggesting that this is a strategy to improve peptaibols production inTrichodermafungi. These results demonstrate the important role of LAE1 in the regulation of peptaibols production inT. longibrachiatumSMF2.