Overexpression of the mango MiCO gene delayed flowering time in transgenic Arabidopsis
Overexpression of the mango MiCO gene delayed flowering time in transgenic Arabidopsis
复制标题
转基因拟南芥中芒果MiCO基因的过度表达延迟了开花时间
DOI：
10.1007/s11240-020-01894-3
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发表时间：
2020-07-26
影响因子：
3
通讯作者：
He, Xin-Hua
中科院分区：
文献类型：
--
作者：
Liu, Yuan;Luo, Cong;He, Xin-Hua
CONSTANS（CO）/CONSTANS-like（COL）基因在光周期开花途径中起重要作用。然而，CO/COL基因在芒果（Mangifera indicaL.）仍然未知。本研究从芒果中分离了MiCO基因并对其进行了功能鉴定。该蛋白的开放阅读框长966 bp，编码322个氨基酸，含有两个保守的B-box锌指结构域和一个CCT结构域，属于CO/COL Ⅰ组蛋白家族。MiCO蛋白与绿色荧光蛋白融合，定位于细胞核，在所有供试器官中均有表达，尤其在非开花枝条的叶和茎中表达量较高，而在开花枝条中表达量较低。从营养生长向生殖生长过渡期间，MiCO的表达量显著增加，且表达量与昼夜节律一致，在09：00时表达量最高。在长日照和短日照条件下，过表达的MiCO inclusionopsis都能显著延迟开花。关键信息在这项研究中，对芒果的aMiCO基因进行了表征。过表达MiCO延迟转基因拟南芥的开花MiCO基因可能在芒果开花过程中起重要作用。
CONSTANS(CO)/CONSTANS-like(COL) genes play an important role in the photoperiodic flowering pathway. However, the functional roles of theCO/COLgenes in mango (Mangifera indicaL.) remain unknown. In this study, theMiCOgene was isolated from mango and functionally characterized. The open reading frame is 966 bp in length and encodes 322 amino acids; the protein contains two conserved B-box zinc finger domains and a CCT domain and thus belongs to the CO/COL group I protein family. The MiCO protein fused to green fluorescent protein localized to the nucleus.MiCOwas expressed in all tested organs and was highly expressed especially in the leaves and stems of nonflowering branches, but its expression decreased in flowering branches. The expression level ofMiCOsignificantly increased during the transition from vegetative to reproductive growth.MiCOwas also expressed in accordance with the circadian rhythm, with the greatest expression level detected at 09:00. Overexpression ofMiCOinArabidopsissignificantly delayed flowering under both long-day and short-day conditions.Key message In this study, aMiCOgene from mango was characterized. Overexpression ofMiCOdelayed flowering in transgenicArabidopsis. TheMiCOgene may play an important role in the flowering process of mango.