A NOVEL CYTOSOLIC COMPONENT, P40(PHOX), OF RESPIRATORY BURST OXIDASE ASSOCIATES WITH P67(PHOX) AND IS ABSENT IN PATIENTS WITH CHRONIC GRANULOMATOUS-DISEASE WHO LACK P67(PHOX)
A NOVEL CYTOSOLIC COMPONENT, P40(PHOX), OF RESPIRATORY BURST OXIDASE ASSOCIATES WITH P67(PHOX) AND IS ABSENT IN PATIENTS WITH CHRONIC GRANULOMATOUS-DISEASE WHO LACK P67(PHOX)
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DOI：
10.1006/bbrc.1994.1383
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发表时间：
1994-03-30
影响因子：
3.1
通讯作者：
KURATSUJI, T
中科院分区：
文献类型：
--
作者：
TSUNAWAKI, S;MIZUNARI, H;KURATSUJI, T
人嗜中性粒细胞的胞质组分p47 phox和p67 phox，其缺陷导致慢性肉芽肿病（CGD），在细胞刺激时增强呼吸爆发氧化酶从胞质移位到膜。在这份报告中，我们描述了一种新的胞质组分，p40 phox，这一贯的行为与p67 phox通过免疫沉淀和柱工程，并在CGD患者缺乏p67 phox失踪。虽然肌动蛋白已被报道参与O2-的产生，p40 phox的配置文件并不对应的肌动蛋白。p40 phox和p67 phox之间的紧密联系不受脱氧胆酸盐和Nonidet P-40的混合物处理的影响，直到进行SDS-PAGE。添加重组p67 phox的胞质溶胶中没有产生任何额外的p40 phox的免疫沉淀，不像添加剂的p67 phox带的增量。这些结果表明，p40 phox与p67 phox以1：1的摩尔比形成复合物，在胞质溶胶中没有任何游离的p40 phox。 (C)1994年出版社出版。
Cytosolic components of human neutrophils, p47phox and p67phox, deficiencies of which lead to chronic granulomatous disease (CGD), potentiate respiratory burst oxidase translocating from cytosol to membrane upon cell stimulation. In this report we describe a novel cytosolic component, p40phox, which consistently behaves with p67phox through immunoprecipitation and column works, and is missing in patients with CGD who lack p67phox. Although actin has been reported to be involved in O2- generation, the p40phox profile did not correspond to that of actin. The tight association between p40phox and p67phox was not affected by treatment with a mixture of deoxycholate and Nonidet P-40, until subjected to SDS-PAGE. Addition of recombinant p67phox to cytosol did not produce any additional p40phox in the immunoprecipitate, unlike the additive increment in the band of p67phox. These results suggest that p40phox forms a complex with p67phox in a molar ratio of 1:1, without any free p40phox in the cytosol. (C) 1994 Academic Press, Inc.