SINGLE GENE DETERMINES HOST RANGE OF INFLUENZA-VIRUS
SINGLE GENE DETERMINES HOST RANGE OF INFLUENZA-VIRUS
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DOI：
10.1038/270617a0
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发表时间：
1977-01-01
期刊：
影响因子：
64.8
通讯作者：
ALMOND, JW
中科院分区：
文献类型：
--
作者：
ALMOND, JW
通过聚丙烯酰胺凝胶电泳确定，流感病毒的基因组由八个不同的单链 RNA 片段组成1-3。这些片段中的每一个都编码八种病毒特有的多肽4-9中的一种，因此被认为是通过单顺反子信使RNA10发挥作用的单个基因。由于不同流感毒株的碱基序列同源性 9 或其 RNA 片段和感染细胞多肽 11,12 的电泳迁移率可能有所不同，因此通常可以确定株间重组体中每个基因的亲本起源。此类程序已用于研究与某些流感病毒特定蛋白质相关的生理特性9,13,14。我在这里描述了这种方法在宿主范围遗传控制研究中的应用。结果表明，两种禽流感病毒宿主范围的差异仅取决于与 RNA 聚合酶活性相关的内部病毒蛋白之一的特性。
THE genome of influenza virus consists of eight distinct segments of single stranded RNA as determined by electrophoresis in polyacrylamide gels1–3. Each of these segments codes for one of the eight virus specified polypeptides4–9and is therefore believed to be a single gene which functions by a monocistronic messenger RNA10. Because different strains of influenza may vary in base sequence homology9or in the electrophoretic mobilities of their RNA segments and infected cell polypeptides11,12, it is often possible to determine the parental origin of every gene in an interstrain recombinant. Such procedures have been used to investigate the physiological properties associated with some of the influenza virus specified proteins9,13,14. I describe here the application of this approach to an investigation into the genetic control of host range. The results show that the difference in host range between two avian influenza viruses depends solely on the properties of one of the internal viral proteins associated with RNA polymerase activity.