Phosphorylation of the nuclear lamins during interphase and mitosis.
Phosphorylation of the nuclear lamins during interphase and mitosis.
复制标题
间期和有丝分裂期间核纤层蛋白的磷酸化。
DOI：
--
复制
发表时间：
1985
影响因子：
4.8
通讯作者：
L. Gerace
中科院分区：
文献类型：
--
作者：
Y. Ottaviano;L. Gerace
核纤层是一种聚合蛋白组装体，被认为充当核膜的结构框架。先前的研究表明，核纤层（“核纤层蛋白”）的主要多肽的磷酸化可能在有丝分裂过程中诱导该结构的分解。为了进一步研究磷酸化在核纤层结构调节中的可能参与，我们表征了哺乳动物组织培养细胞在间期和有丝分裂期间发生的核纤层蛋白磷酸化。磷酸化在整个间期持续发生（与核膜生长一致），并且主要发生在组装的核纤层上。当细胞分裂过程中核纤层被分解时，核纤层会被大约 1-2 个相关磷酸盐分子修饰。该有丝分裂磷酸化水平比平均间期水平高 4-7 倍。磷酸核纤层蛋白主要以磷酸丝氨酸的形式存在，并分布在许多胰蛋白酶肽中，其中许多在间期和有丝分裂期都被修饰。值得注意的是，磷酸化是唯一可检测到的核纤层蛋白的电荷改变合成后修饰，专门发生在有丝分裂期间。这项研究的结果支持磷酸化对于间期和有丝分裂层结构的调节很重要的观点。
The nuclear lamina is a polymeric protein assembly that is proposed to function as an architectural framework for the nuclear envelope. Previous work suggested that phosphorylation of the major polypeptides of the lamina (the "lamins") may induce disassembly of this structure during mitosis. To further investigate the possible involvement of phosphorylation in regulation of lamina structure, we characterized lamin phosphorylation occurring in mammalian tissue culture cells during interphase and mitosis. Phosphorylation occurs continuously throughout all interphase periods (coordinately with nuclear envelope growth), and takes place mainly on the assembled lamina. When the lamina is disassembled during cell division, the lamins are modified with approximately 1-2 molecules of associated phosphate. This level of mitotic phosphorylation is 4-7-fold higher than the average interphase level. Lamin phosphate occurs predominantly as phosphoserine, and is distributed over numerous tryptic peptides, many of which are modified during both interphase and mitotic periods. Significantly, phosphorylation is the only detectable charge-altering postsynthetic modification of the lamins that occurs specifically during mitosis. The results of this study support the notion that phosphorylation is important for regulation of interphase and mitotic lamina structure.