Identification of functional marker proteins in the mammalian growth cone
Identification of functional marker proteins in the mammalian growth cone
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DOI：
10.1073/pnas.0904092106
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发表时间：
2009-10-06
影响因子：
11.1
通讯作者：
Igarashi, Michihiro
中科院分区：
文献类型：
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作者：
Nozumi, Motohiro;Togano, Tetsuya;Igarashi, Michihiro
哺乳动物生长锥中蛋白质的鉴定有可能促进我们对神经元生长和神经回路形成的关键调节剂的理解;然而，迄今为止，只有一种生长锥标记蛋白GAP-43被报道。在这里，我们成功地使用蛋白质组学的方法来确定945蛋白存在于发育中的大鼠前脑生长锥，包括高度丰富的，膜相关和肌动蛋白相关的蛋白质。通过定量免疫染色确定，几乎100种蛋白质似乎在生长锥中高度富集，并且对于17种蛋白质，RNAi的结果表明在轴突生长中的作用。我们发现的大多数蛋白质以前没有涉及轴突生长，因此它们的鉴定向前迈出了重要的一步，提供了标记蛋白和候选神经元生长相关蛋白。
Identification of proteins in the mammalian growth cone has the potential to advance our understanding of this critical regulator of neuronal growth and formation of neural circuit; however, to date, only one growth cone marker protein, GAP-43, has been reported. Here, we successfully used a proteomic approach to identify 945 proteins present in developing rat forebrain growth cones, including highly abundant, membrane-associated and actin-associated proteins. Almost 100 of the proteins appear to be highly enriched in the growth cone, as determined by quantitative immunostaining, and for 17 proteins, the results of RNAi suggest a role in axon growth. Most of the proteins we identified have not previously been implicated in axon growth and thus their identification presents a significant step forward, providing marker proteins and candidate neuronal growth-associated proteins.