Characterization of a translation inhibitory protein from Luffa aegyptiaca.
Characterization of a translation inhibitory protein from Luffa aegyptiaca.
复制标题
丝瓜翻译抑制蛋白的表征。
DOI：
10.1016/0006-291x(89)92462-5
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发表时间：
1989
影响因子：
3.1
通讯作者：
Xu,FJ
中科院分区：
文献类型：
--
作者：
Ramakrishnan,S;Enghlid,JJ;BryantJr,HL;Xu,FJ
A protein with a molecular weight of about 30,000 was purified from the seeds ofLuffa aegyptiaca. This protein inhibited cell free translation at pM concentrations. In spite of functional similarity to other ribosomal inhibitory proteins, the NH2-terminal analysis did not show any significant homology. Competitive inhibition studies indicate no immunological crossreactivity between the inhibitory protein fromLuffa aegyptiaca, pokeweed antiviral protein (PAP) and recombinant ricin A chain. Chemical linkage of the protein to a monoclonal antibody reactive to transferrin receptor resulted in a highly cytotoxic conjugate.
