Optisol vs Dexsol as storage media for preservation of human corneal epithelium
Optisol vs Dexsol as storage media for preservation of human corneal epithelium
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DOI：
10.1038/sj.eye.6700693
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发表时间：
2004-05-01
期刊：
影响因子：
3.9
通讯作者：
Rootman, D
中科院分区：
文献类型：
--
作者：
Greenbaum, A;Hasany, SM;Rootman, D
目的比较OptisolGS和Dexsol两种保存液保存供体角膜上皮的效果，方法选择12对不适合移植的角膜，其中1对保存于OptisolGS中，另1对保存于Dexsol中。在三个储存持续时间- 1、2和4天F中的每一个，制备这些成对角膜中的四个用于光学显微镜和扫描及透射电子显微镜。另外四对对照角膜以相同的方式制备，并放置在通用fixation.Main结果measurements评价的角膜是由两个观察者蒙上身份的存储介质和存储长度。通过光学显微镜评价上皮细胞的损失。上皮细胞的基底膜，细胞的完整性，细胞间连接，和细胞内细胞器的附着进行了评价和比较，通过电子显微镜。结果上皮损失的大小与存储时间的长度。对照角膜维持正常上皮，保留所有上皮细胞层。保存1天的角膜上皮损伤最小。保存2天的角膜上皮损伤轻微增加，保存4天的角膜上皮损伤显著增加。两种存储介质之间没有显著差异。基底细胞层保持在两个媒体在所有的时间点，通常在良好的条件下与轻度至中度的损害，在某些cases.Conclusions供体上皮细胞的损失主要是相关的存储长度，是类似的Optisol GS和Dexsol。保存时间应少于4天，特别是对眼表疾病患者进行穿透性角膜移植术时。
Purpose To compare the efficacy of two storage media, Optisol GS and Dexsol, in preservation of donor corneal epithelium.Methods A total of 12 pairs of corneas not suitable for transplantation, all with intact epithelium, were used in this study, with one cornea of the pair stored in Optisol GS and its other counterpart in Dexsol. At each of three durations of storage - 1, 2, and 4 days F four of these paired corneas were prepared for light microscopy and scanning and transmission electron microscopy. Another four pairs of control cornea were prepared in the same way and placed in universal fixative.Main outcome measures Evaluation of the corneas was made by two observers masked as to the identity of the storage medium and length of storage. Loss of epithelial cells was evaluated by light microscopy. The attachment of the epithelium to the basement membrane, cellular integrity, intercellular junctions, and intracellular organelles were evaluated and compared by electron microscopy.Results The magnitude of epithelial loss correlated with the length of storage time. Control corneas maintained normal epithelium with preservation of all epithelial cell layers. Corneas stored for 1 day had minimal damage of the epithelium. Corneas stored for 2 days had a slight increase in epithelial damage, and corneas stored for up to 4 days showed a marked increase in epithelial damage. There were no significant differences between the two storage media. The basal cell layer was maintained in both the media at all time points, usually in good condition with mild-to-moderate damage in some cases.Conclusions Loss of donor epithelium is related mainly to the length of storage and is similar in both Optisol GS and Dexsol. The storage time should be less than 4 days, especially when performing penetrating keratoplasty on patients with ocular surface disorders.