Morphogenesis of T-tubules in heart cells: the role of junctophilin-2
Morphogenesis of T-tubules in heart cells: the role of junctophilin-2
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DOI：
10.1007/s11427-013-4490-4
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发表时间：
2013-07-01
影响因子：
9.1
通讯作者：
Wang ShiQiang
中科院分区：
文献类型：
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作者：
Han Jing;Wu HaoDi;Wang ShiQiang
T-小管（TT）系统形成心脏和肌肉细胞中兴奋-收缩偶联的结构基础。TT系统的形态发生是心脏细胞成熟的关键步骤，因为它不存在于新生心肌细胞中。在本研究中，我们量化了心脏细胞成熟过程中TT的形态学变化，并研究了嗜连接蛋白2（JP 2）的作用，该蛋白是一种已知的将肌浆网（SR）锚到TT的蛋白质，在TT形态学参数的变化中。共聚焦图像分析表明，TTs成熟过程中横向成分增加，而纵向成分减少。傅立叶变换分析显示，随着心肌细胞的成熟，近似2 μ m的空间分量的功率增加。这些变化之前增加的JP 2的表达，并逆转JP 2敲低。这些发现表明，JP 2是心脏发育过程中TT形态发生所必需的。
The T-tubule (TT) system forms the structural basis for excitation-contraction coupling in heart and muscle cells. The morphogenesis of the TT system is a key step in the maturation of heart cells because it does not exist in neonatal cardiomyocytes. In the present study, we quantified the morphological changes in TTs during heart cell maturation and investigated the role of junctophilin-2 (JP2), a protein known to anchor the sarcoplasmic reticulum (SR) to TT, in changes to TT morphological parameters. Analysis of confocal images showed that the transverse elements of TTs increased, while longitudinal elements decreased during the maturation of TTs. Fourier transform analysis showed that the power of similar to 2 mu m spatial components increased with cardiomyocytes maturation. These changes were preceded by increased expression of JP2, and were reversed by JP2 knockdown. These findings indicate that JP2 is required for the morphogenesis of TTs during heart development.