Comprehensive analysis of lncRNA and mRNA expression profiles in macrophages activated by Actinidia eriantha polysaccharide
Comprehensive analysis of lncRNA and mRNA expression profiles in macrophages activated by Actinidia eriantha polysaccharide
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毛花猕猴桃多糖激活巨噬细胞lncRNA和mRNA表达谱综合分析
DOI：
10.1016/j.ijbiomac.2019.06.091
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发表时间：
2019-09-01
影响因子：
8.2
通讯作者：
Sun, Hongxiang
中科院分区：
文献类型：
--
作者：
Chen, Xiangfeng;Du, Jing;Sun, Hongxiang
猕猴桃根多糖(Actinidia eriantha，AEPS)是一种有效的抗肿瘤和免疫佐剂。最近的证据表明，长非编码RNA(LncRNAs)在调节免疫细胞的激活和功能方面发挥着重要作用。本研究的目的是利用全基因表达芯片研究lncRNAs和mRNAs在AEPS激活的RAW264.7细胞中的表达谱，并探讨其在RAW264.7细胞中的作用。AEP诱导RAW264.7细胞中1,807和506个lncRNAs的差异表达。AEPS可显著上调RAW264.7细胞M1和M2特异性细胞因子和趋化因子的mRNA表达水平。核因子-kappa B抑制剂和shRNA-核因子-kappaB p65显著抑制IL-1β的上调。AEPS诱导RAW264.7细胞表达。5个AEPS诱导的lncRNAs(D730047E02Rik、Gm14047、A30_P01020139、A30_P01026293和A30_P01032196)通过与核因子-kappa B p65的特异性相互作用，在RAW264.7细胞中局部调控免疫应答基因的表达。抑制核因子-kappaB可显著抑制AEPS诱导的这些lncRNAs的表达。这些结果表明，AEPS通过lncRNAs/NF-kappa B网络诱导巨噬细胞活化。本研究进一步拓展了目前对植物多糖作用机理的认识。(C)2019爱思唯尔B.V.保留所有权利。
The polysaccharide from the roots of Actinidia eriantha (AEPS) is a potent antitumor agent and immunological adjuvant. The recent evidences indicate that long noncoding RNAs (lncRNAs) play essential roles in regulating the activation and function of immune cells. The objective of this study was to investigate the expression profiles of lncRNAs and mRNAs and explore the role of lncRNAs in AEPS-activated RAW264.7 cells using whole gene expression microarray. AEPS induced the differential expression of 1,807 mRNAs and 506 lncRNAs in RAW264.7 cells. The mRNA expression levels of both M1 and M2 specific cytokines and chemokines were significantly upregulated in RAW264.7 cells by AEPS. NF-kappa B inhibitors and shRNA-NF-kappa B p65 significantly decreased the up regulation of IL-1 beta. expression in RAW264.7 cells induced by AEPS. Five AEPS-induced lncRNAs (D730047E02Rik, Gm14047, A_30_P01020139, A_30_P01026293 and A30_P01032196) were identified and predicted to locally regulate mRNA expressions of immune response genes in RAW264.7 cells through the specific interaction with NF-kappa B p65. The inhibition of NF-kappa B could significantly suppress the expression of these lncRNAs induced by AEPS. These results indicated that AEPS induced the activation of macrophages via lncRNAs/NF-kappa B networks. This study further expanded current knowledge on the mechanisms of plant polysaccharides. (C) 2019 Elsevier B.V. All rights reserved.