Analysis of alpha-particle induced chromosome aberrations in human lymphocytes, using pan-centromeric and pan-telomeric probes.
Analysis of alpha-particle induced chromosome aberrations in human lymphocytes, using pan-centromeric and pan-telomeric probes.
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使用泛着丝粒和泛端粒探针分析α粒子诱导的人类淋巴细胞染色体畸变。
DOI：
10.1080/09553000400017416
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发表时间：
2004
影响因子：
2.6
通讯作者：
Barquinero,JF
中科院分区：
文献类型：
--
作者：
Mestres,M;Caballin,MR;Schmid,E;Stephan,G;Sachs,R;Barrios,L;Barquinero,JF
目的：本研究的目的是通过同时检测人淋巴细胞中存在的所有着丝粒和端粒，评价α粒子辐照后诱导的不完全染色体畸变。材料与方法：用~（241）Am放射源分别以0.2、0.5、0.7和1戈伊剂量照射贴壁淋巴细胞。使用泛着丝粒和泛端粒探针应用荧光素原位杂交（FISH）技术。使用Cytovision®FISH工作站对所有异常细胞进行数字化和分析。结果：共检出378条不完全染色体和不完全无着丝粒，未检出端粒大于92个的病例。总不完全元素与多中心元素的比值为1.00。无着丝粒（ace）片段总数为822个;其中57%为完整片段ace（+，+），26%为不完整片段ace（+，−），17%为间质片段ace（−，−）;平均相对长度分别为2.91±0.06、1.91±0.07和1.63±0.07。在所有三种情况下，在长度分布中均发现了第二个峰，对应于3.5和4之间的相对长度。结论：α粒子暴露后不完全重新连接的百分比高于先前描述的低线性能量转移（LET）辐射暴露。结果似乎表明，与低LET辐射相比，α粒子暴露后，含着丝粒的元素更有可能被修复。许多间隙碎片是大的线性形式，不能被认为是不可区分的无中心环。
Purpose: The aim of the present study has been the evaluation of the incomplete chromosome aberrations induced afterα‐particle irradiation by the simultaneous detection of all centromeres and telomeres present in human lymphocytes. Moreover, a study on the lengths of the different acentric fragments is presented.Materials and methods: Attached lymphocytes were irradiated at doses of 0.2, 0.5, 0.7 and 1 Gy using a241Am source. Flourescent in‐situ hybridization (FISH) techniques were applied using pan‐centromeric and pan‐telomeric probes. All abnormal cells were digitalised and analysed using a Cytovision®FISH workstation. The description of all abnormalities observed, and the length of the acentric fragments was recorded.Results: A total of 378 incomplete chromosomes plus incomplete acentrics was found. Cases with more than 92 telomeres were not detected. The ratio between total incomplete elements and multicentrics was 1.00. The total number of acentric (ace) fragments was 822; 57% of them were complete fragments ace (+,+), 26% incomplete fragments ace (+,−), and 17% interstitial fragments ace(−,−); the mean relative lengths were 2.91±0.06, 1.91±0.07 and 1.63±0.07, respectively. In all three cases a secondary peak in the length distribution was found, corresponding to a relative length between 3.5 and 4.Conclusion: The percentage of incomplete rejoinings is higher afterα‐particle exposure than that described previously for low‐linear energy transfer (LET) radiation exposures. The results seem to indicate that compared to low‐LET radiation, afterα‐particle exposure centromere‐containing elements are more likely to be repaired. Many interstitial fragments are large linear forms that cannot be considered as non‐distinguishable acentric rings.