Dietary supplementation with soy isoflavones or replacement with soy proteins prevents hepatic lipid droplet accumulation and alters expression of genes involved in lipid metabolism in rats
Dietary supplementation with soy isoflavones or replacement with soy proteins prevents hepatic lipid droplet accumulation and alters expression of genes involved in lipid metabolism in rats
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膳食补充大豆异黄酮或用大豆蛋白替代可防止大鼠肝脏脂滴积累并改变参与脂质代谢的基因表达
DOI：
10.1007/s12263-013-0373-3
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发表时间：
2013
影响因子：
3.5
通讯作者：
K. Cockell
中科院分区：
文献类型：
--
作者：
C. Xiao;Carla M. Wood;D. Weber;S. Aziz;R. Mehta;P. Griffin;K. Cockell
肝脂滴（HLD）的积聚是非酒精性脂肪性肝病（NAFLD）的标志性病理。本研究探讨了大豆异黄酮（ISF）和不同量的大豆蛋白对大鼠高脂血症、脂质代谢及相关基因表达的影响。给断奶Sprague-Dawley大鼠喂食含有20%酪蛋白的饲料，不含（D1）或含（D2）补充ISF（50 mg/kg饲料）或用增加量的醇洗大豆分离蛋白（SPI，5、10和20%; D3、D4、D5）替代酪蛋白，持续90天。酪蛋白（20%）可引起雌性大鼠肝脂蛋白胆固醇（HLD）的蓄积，但对雄性大鼠无此作用.大豆蛋白和ISF均能显著抑制HLD的形成。大豆蛋白以剂量依赖性方式降低肝脏总胆固醇和甘油三酯。有趣的是，与D1相比，大豆蛋白而不是ISF显著增加了雌性大鼠肝脏中的游离脂肪酸。蛋白质组学分析表明，大豆蛋白在D1组中下调了至少3种脂肪生成相关酶的表达，上调了7种脂肪酸β-氧化或脂解相关蛋白的表达。另外，9个差异表达蛋白与氨基酸代谢相关，5个与糖酵解相关，2个与胆固醇代谢相关。膳食ISF和SPI显著降低雌性大鼠肝过氧化物酶体增殖物激活受体γ2（PPARγ2）和脂肪特异性蛋白27（FSP 27）。总的来说，这项研究表明，用大豆蛋白或补充大豆ISF部分或全部替代膳食酪蛋白可以有效预防HLD的积累。其机制可能与抑制脂肪生成、促进脂肪分解、下调PPARγ2和FSP 27有关。这表明食用大豆食品或补充剂可能是预防或治疗脂肪肝疾病的有用策略。
Accumulation of hepatic lipid droplet (HLD) is the hallmark pathology of non-alcoholic fatty liver disease (NAFLD). This study examined the effects of soy isoflavones (ISF) and different amounts of soy proteins on the accumulation of HLD, lipid metabolism and related gene expression in rats. Weanling Sprague–Dawley rats were fed diets containing either 20 % casein protein without (D1) or with (D2) supplemental ISF (50 mg/kg diet) or substitution of casein with increasing amounts of alcohol-washed soy protein isolate (SPI, 5, 10, and 20 %; D3, D4, D5) for 90 days. Dietary casein (20 %) induced accumulation of HLD in female, but not in male rats. Both soy proteins and ISF remarkably prevented the formation of HLD. Soy proteins lowered hepatic total cholesterol and triglyceride in a dose-dependent manner. Interestingly, soy proteins but not ISF significantly increased free fatty acids in the liver of the female rats compared to D1. Proteomic analysis showed that at least 3 enzymes involved in lipogenesis were down-regulated and 7 proteins related to fatty acid β-oxidation or lipolysis were up-regulated by soy protein over D1. Additionally, 9 differentially expressed proteins identified were related to amino acid metabolism, 5 to glycolysis and 2 to cholesterol metabolism. Dietary ISF and SPI markedly reduced hepatic-peroxisome-proliferator-activated receptor γ2 (PPARγ2) and fat-specific protein 27 (FSP27) in female rats. Overall, this study has shown that partial or full replacement of dietary casein by soy protein or supplementation with soy ISF can effectively prevent the accumulation of HLD. The potential molecular mechanism(s) involved might be due to suppression of lipogenesis and stimulation of lipolysis and down-regulation of PPARγ2 and FSP27. This suggests that consumption of soy foods or supplements might be a useful strategy for the prevention or treatment of fatty liver diseases.
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完整的膳食大豆蛋白，但未在酪蛋白中添加富含异黄酮的大豆提取物，可以改善切除卵巢的食蟹猴的血浆脂质。
DOI：
10.1093/jn/129.8.1585
发表时间：
1999
期刊：
The Journal of nutrition
影响因子：
--
作者：
Greaves,KA;Parks,JS;Williams,JK;Wagner,JD
通讯作者：
Wagner,JD
影响因子：
29
作者：
Matsusue, Kimihiko;Kusakabe, Takashi;Gonzalez, Frank J.
通讯作者：
Gonzalez, Frank J.
影响因子：
15.9
作者：
VidalPuig, A;JimenezLinan, M;Moller, DE
通讯作者：
Moller, DE
影响因子：
4.2
作者：
Adams, MR;Golden, DL;Williams, JK
通讯作者：
Williams, JK