Quantitative 1H NMR spectroscopy
Quantitative 1H NMR spectroscopy
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DOI：
10.1016/j.trac.2012.02.007
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发表时间：
2012-05-01
影响因子：
13.1
通讯作者：
Roy, Raja
中科院分区：
文献类型：
--
作者：
Bharti, Santosh Kumar;Roy, Raja
本文综述了核磁共振（NMR）谱用于小分子的定量分析的必要性，介绍了定量NMR（qNMR）的基本概念，简要描述了获得高质量、可重复的NMR谱的重要采集和处理参数，以最大限度地提高准确度，以及用于定量分析的最新参考技术。我们还描述了用于定量分析的方法，包括校准曲线法和标准加入法。此外，我们简要地讨论了qNMR光谱的验证及其在各个科学学科中的主要应用。(C)2012爱思唯尔有限公司保留所有权利。
This review illustrates the need to use nuclear magnetic resonance (NMR) spectroscopy for the quantitative analysis of small molecules in their crude forms and in mixtures.We provide the basic concepts of quantitative NMR (qNMR), a brief description of important acquisition and processing parameters responsible for obtaining high-quality, reproducible NMR spectra in order to maximize accuracy, and the latest referencing techniques used for quantitative analysis.We also describe methods that are used for quantitative analysis including calibration-curve and standard-addition methods. Further, we briefly address validation of qNMR spectroscopy and its major applications in various scientific disciplines. (C) 2012 Elsevier Ltd. All rights reserved.