Testing DNA barcoding in closely related groups of Lysimachia L. (Myrsinaceae)
Testing DNA barcoding in closely related groups of Lysimachia L. (Myrsinaceae)
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在密切相关的金钱草属（紫金牛科）群体中测试 DNA 条形码
DOI：
10.1111/j.1755-0998.2011.03076.x
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发表时间：
2012-01-01
影响因子：
7.7
通讯作者：
Ge, Xue-Jun
中科院分区：
文献类型：
--
作者：
Zhang, Cai-Yun;Wang, Feng-Ying;Ge, Xue-Jun
有人认为rbcL和matK是植物中的核心条形码，但它们的功能不足以区分密切相关的植物群。需要评估额外的条形码以提高植物物种之间的区分水平。由于它们的分类学和极端的多样性，我们使用中国的过路黄属（Myrsinaceae）物种来测试核心条形码（rbcL和matK）和两个额外的候选条形码（trnH-psbA和核核糖体ITS）的性能;来自四个亚属的97份材料代表34个假定的过路黄属物种被纳入本研究。亚属中有许多密切相关的物种配对。将过路黄覆盖以检测其辨别力。在这项研究中，rbcL和matK单独或组合在密切相关的植物组中的无效性得到验证。TrnH-psbA与rbcL + matK组合在金钱草组中表现不佳。 与此相反，ITS，单独或与rbcL和/或matK相结合，显示高分辨率的能力，在过路黄。我们支持ITS作为核心条形码rbcL和matK基础上的补充条形码。此外，本研究还阐明了DNA条形码检测到的几个错误或潜在的进化事件。
It has been suggested that rbcL and matK are the core barcodes in plants, but they are not powerful enough to distinguish between closely related plant groups. Additional barcodes need to be evaluated to improve the level of discrimination between plant species. Because of their well‐studied taxonomy and extreme diversity, we used Chinese Lysimachia (Myrsinaceae) species to test the performance of core barcodes (rbcL and matK) and two additional candidate barcodes (trnH‐psbA and the nuclear ribosomal ITS); 97 accessions from four subgenus representing 34 putative Lysimachia species were included in this study. And many closely related species pairs in subgen. Lysimachia were covered to detect their discriminatory power. The inefficiency of rbcL and matK alone or combined in closely related plant groups was validated in this study. TrnH‐psbA combined with rbcL + matK did not yet perform well in Lysimachia groups. In contrast, ITS, alone or combined with rbcL and/or matK, revealed high resolving ability in Lysimachia. We support ITS as a supplementary barcode on the basis of core barcode rbcL and matK. Besides, this study also illustrates several mistakes or underlying evolutionary events in Lysimachia detected by DNA barcoding.