The Syrian hamster as a model for the dilated cardiomyopathy of Chagas' disease: a quantitative echocardiographical and histopathological analysis
The Syrian hamster as a model for the dilated cardiomyopathy of Chagas' disease: a quantitative echocardiographical and histopathological analysis
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DOI：
10.1016/j.micinf.2003.07.001
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发表时间：
2003-10-01
影响因子：
5.8
通讯作者：
Cunha-Neto, E
中科院分区：
文献类型：
--
作者：
Bilate, AMB;Salemi, VMC;Cunha-Neto, E
慢性南美锥虫病心肌病 (CCC) 是由原生动物克氏锥虫引起的，在拉丁美洲 16-1800 万人中，有 30% 受到感染。仍然需要一种良好的啮齿动物模型，该模型在克氏锥虫感染后发展出与人类 CCC 非常相似的扩张型心肌病。我们使用定量方法将克氏锥虫感染的叙利亚仓鼠发生的心肌病与人类恰加斯病心肌病进行了比较。雌性仓鼠感染 3.5 x 10(4) (G1, n = 10) 或 10(5) (G2, n = 10) 克氏锥虫 Y 株血锥鞭毛体。对照动物（C，n = 10）注射盐水溶液。感染后4、8和12个月通过超声心动图评估心脏功能。感染后 12 个月将心脏切片提交进行组织病理学/形态测量分析。此时，G1 和 G2 感染组中分别有 91% 和 100% 观察到心室功能障碍和弥漫性或多灶性心肌炎。 C、G1和G2组间质胶原体积中位数分别为1.2%、1.9%和3.9%，其中G2组显着高于C组。在感染动物中，心肌炎与间质纤维化呈正相关。慢性期（感染后 8-12 个月）的死亡在 G2 中比 G1 中更常见，并且与肉眼可见的心室扩张、严重心肌炎和纤维化值增加以及心室功能障碍较早发生有关。长期感染克氏锥虫的叙利亚仓鼠会出现一种类似于人类恰加斯病心肌病的心肌病，并且可能是研究这种疾病的致病机制和寻找新的治疗策略的充分工具。 (C) 2003 年科学与医学版 Elsevier SAS。版权所有。
Chronic Chagas' disease cardiomyopathy (CCC) is caused by the protozoan Trypanosoma cruzi, and it affects 30% of the 16-18 million people infected in Latin America. A good rodent model that develops a dilated cardiomyopathy closely resembling human CCC after T cruzi infection is still needed. We compared the cardiomyopathy developed by T cruzi-infected Syrian hamsters with human Chagas' disease cardiomyopathy using quantitative methods. Female hamsters were infected with 3.5 x 10(4) (G1, n = 10) or 10(5) (G2, n = 10) T. cruzi Y strain blood trypomastigotes. Control animals (C, n = 10) were injected with saline solution. Cardiac function was assessed by echocardiography at 4, 8 and 12 months post-infection. Heart sections were submitted to histopathological/morphometric analysis 12 months post-infection. At this time, ventricular dysfunction and diffuse or multi-focal myocarditis were observed in 91% and 100% of G1 and G2 infected groups, respectively. Median interstitial collagen volumes in groups C, G1 and G2 were 1.2%, 1.9% and 3.9%, respectively, and were significantly higher in group G2 than in group C. Among infected animals, myocarditis showed a positive correlation with interstitial fibrosis. Deaths in the chronic phase (8-12 months post-infection) were more frequent among G2 than G1, and were associated with macroscopic ventricular dilation, severe myocarditis and increased fibrosis values, along with an earlier onset of ventricular dysfunction. The T cruzi chronically infected Syrian hamster develops a cardiomyopathy which resembles human Chagas' disease cardiomyopathy, and might be an adequate tool to investigate pathogenic mechanisms of this disease and to search for novel therapeutic strategies. (C) 2003 Editions scientifiques et medicales Elsevier SAS. All rights reserved.