A physical sciences network characterization of non-tumorigenic and metastatic cells.
A physical sciences network characterization of non-tumorigenic and metastatic cells.
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DOI：
10.1038/srep01449
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发表时间：
2013
影响因子：
4.6
通讯作者：
Wu, Pei-Hsun
中科院分区：
文献类型：
--
作者：
Agus, David B.;Alexander, Jenolyn F.;Arap, Wadih;Ashili, Shashanka;Aslan, Joseph E.;Austin, Robert H.;Backman, Vadim;Bethel, Kelly J.;Bonneau, Richard;Chen, Wei-Chiang;Chen-Tanyolac, Chira;Choi, Nathan C.;Curley, Steven A.;Dallas, Matthew;Damania, Dhwanil;Davies, Paul C. W.;Decuzzi, Paolo;Dickinson, Laura;Estevez-Salmeron, Luis;Estrella, Veronica;Ferrari, Mauro;Fischbach, Claudia;Foo, Jasmine;Fraley, Stephanie I.;Frantz, Christian;Fuhrmann, Alexander;Gascard, Philippe;Gatenby, Robert A.;Geng, Yue;Gerecht, Sharon;Gillies, Robert J.;Godin, Biana;Grady, William M.;Greenfield, Alex;Hemphill, Courtney;Hempstead, Barbara L.;Hielscher, Abigail;Hillis, W. Daniel;Holland, Eric C.;Ibrahim-Hashim, Arig;Jacks, Tyler;Johnson, Roger H.;Joo, Ahyoung;Katz, Jonathan E.;Kelbauskas, Laimonas;Kesselman, Carl;King, Michael R.;Konstantopoulos, Konstantinos;Kraning-Rush, Casey M.;Kuhn, Peter;Kung, Kevin;Kwee, Brian;Lakins, Johnathon N.;Lambert, Guillaume;Liao, David;Licht, Jonathan D.;Liphardt, Jan T.;Liu, Liyu;Lloyd, Mark C.;Lyubimova, Anna;Mallick, Parag;Marko, John;McCarty, Owen J. T.;Meldrum, Deirdre R.;Michor, Franziska;Mumenthaler, Shannon M.;Nandakumar, Vivek;O'Halloran, Thomas V.;Oh, Steve;Pasqualini, Renata;Paszek, Matthew J.;Philips, Kevin G.;Poultney, Christopher S.;Rana, Kuldeepsinh;Reinhart-King, Cynthia A.;Ros, Robert;Semenza, Gregg L.;Senechal, Patti;Shuler, Michael L.;Srinivasan, Srimeenakshi;Staunton, Jack R.;Stypula, Yolanda;Subramanian, Hariharan;Tlsty, Thea D.;Tormoen, Garth W.;Tseng, Yiider;van Oudenaarden, Alexander;Verbridge, Scott S.;Wan, Jenny C.;Weaver, Valerie M.;Widom, Jonathan;Will, Christine;Wirtz, Denis;Wojtkowiak, Jonathan;Wu, Pei-Hsun
为了从物理科学的角度研究从非癌到转移性的转变，物理科学-肿瘤学中心(PS-OC)网络对非致癌MCF-10A和转移性MDA-MB-231乳腺上皮细胞株进行了分子和生物物理比较研究，这两种细胞系通常被用作癌症转移的模型。实验在12个PS-OCS的20个实验室进行。每个实验室都提供了相同的等量、通用试剂和培养方案。对这些测量结果的分析表明，它们在力学、迁移、粘附性、氧反应和蛋白质组学方面存在显著差异。基于模型的多重组学方法确定了这些细胞在形态和生存方面的调控网络之间的关键差异。这些结果提供了两个广泛使用的细胞系的细胞参数的多方面描述，并证明了PS-OC网络方法在整合各种实验观察以阐明与癌症转移相关的表型方面的价值。
To investigate the transition from non-cancerous to metastatic from a physical sciences perspective, the Physical Sciences–Oncology Centers (PS-OC) Network performed molecular and biophysical comparative studies of the non-tumorigenic MCF-10A and metastatic MDA-MB-231 breast epithelial cell lines, commonly used as models of cancer metastasis. Experiments were performed in 20 laboratories from 12 PS-OCs. Each laboratory was supplied with identical aliquots and common reagents and culture protocols. Analyses of these measurements revealed dramatic differences in their mechanics, migration, adhesion, oxygen response, and proteomic profiles. Model-based multi-omics approaches identified key differences between these cells' regulatory networks involved in morphology and survival. These results provide a multifaceted description of cellular parameters of two widely used cell lines and demonstrate the value of the PS-OC Network approach for integration of diverse experimental observations to elucidate the phenotypes associated with cancer metastasis.
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影响因子：
4.8
作者：
Debnath, J;Muthuswamy, SK;Brugge, JS
通讯作者：
Brugge, JS
影响因子：
2.8
作者：
Akkiprik, Mustafa;Nicorici, Daniel;Zhang, Wei
通讯作者：
Zhang, Wei
影响因子：
4.8
作者：
Balzer, Eric M.;Tong, Ziqiu;Konstantopoulos, Konstantinos
通讯作者：
Konstantopoulos, Konstantinos
影响因子：
3.4
作者：
Lee, Meng-Horng;Wu, Pei-Hsun;Wirtz, Denis
通讯作者：
Wirtz, Denis
粘着斑蛋白在三维细胞运动中的独特作用。
影响因子：
21.3
作者：
Fraley, Stephanie I.;Feng, Yunfeng;Krishnamurthy, Ranjini;Kim, Dong-Hwee;Celedon, Alfredo;Longmore, Gregory D.;Wirtz, Denis
通讯作者：
Wirtz, Denis