Towards the development of a sustainable soya bean-based feedstock for aquaculture.
Towards the development of a sustainable soya bean-based feedstock for aquaculture.
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致力于开发可持续的大豆基本原料用于水产养殖。
DOI：
10.1111/pbi.12608
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发表时间：
2017-02
影响因子：
13.8
通讯作者：
Clemente TE
中科院分区：
文献类型：
--
作者：
Park H;Weier S;Razvi F;Peña PA;Sims NA;Lowell J;Hungate C;Kissinger K;Key G;Fraser P;Napier JA;Cahoon EB;Clemente TE
大豆（甘氨酸max (L.)）)因其油和蛋白质成分而受到追捧。在大豆育种和分子生物学项目中，正在努力通过遗传方法为这两个组成部分增加价值。前者是世界上消费的主要植物油。因此，它的主要用途是人类直接消费。为了增加其在饲料应用中的效用，从而扩大大豆副产品的市场，我们研究了在虹鳟（Oncorhynchus mykiss）和Kampachi （Seriola rivoliana）中，用大豆蛋白和富含α -亚麻酸和硬脂酸的高Omega - 3脂肪酸大豆油同时替代水产饲料中的海洋成分。本文介绍的水产饲料配方主要减少了海洋成分，这意味着收获的肉中有更多的总Omega - 3脂肪酸。在这些发现的基础上，后续的努力指向了一种遗传策略，该策略将转化为一种基于水产养殖的最佳身份保存大豆原料的原型设计，从而将一种多基因堆叠方法引入到作物中，用于有针对性地合成两种增值输出性状，二十碳五烯酸和类酮胡萝卜素虾青素。为此，本文系统地介绍了7个转基因盒式大豆，并对衍生的新事件进行了分子和表型评价。
Soya bean (Glycine max (L.) Merr.) is sought after for both its oil and protein components. Genetic approaches to add value to either component are ongoing efforts in soya bean breeding and molecular biology programmes. The former is the primary vegetable oil consumed in the world. Hence, its primary usage is in direct human consumption. As a means to increase its utility in feed applications, thereby expanding the market of soya bean coproducts, we investigated the simultaneous displacement of marine ingredients in aquafeeds with soya bean‐based protein and a high Omega‐3 fatty acid soya bean oil, enriched with alpha‐linolenic and stearidonic acids, in both steelhead trout (Oncorhynchus mykiss) and Kampachi (Seriola rivoliana). Communicated herein are aquafeed formulations with major reduction in marine ingredients that translates to more total Omega‐3 fatty acids in harvested flesh. Building off of these findings, subsequent efforts were directed towards a genetic strategy that would translate to a prototype design of an optimal identity‐preserved soya bean‐based feedstock for aquaculture, whereby a multigene stack approach for the targeted synthesis of two value‐added output traits, eicosapentaenoic acid and the ketocarotenoid, astaxanthin, were introduced into the crop. To this end, the systematic introduction of seven transgenic cassettes into soya bean, and the molecular and phenotypic evaluation of the derived novel events are described.