OCCURRENCE AND DISTRIBUTION OF THYMINE AND 3 METHYLATED-ADENINE BASES IN RIBONUCLEIC ACIDS FROM SEVERAL SOURCES
OCCURRENCE AND DISTRIBUTION OF THYMINE AND 3 METHYLATED-ADENINE BASES IN RIBONUCLEIC ACIDS FROM SEVERAL SOURCES
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DOI：
10.1042/bj0700642
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发表时间：
1958-01-01
影响因子：
4.1
通讯作者：
DUNN, DB
中科院分区：
文献类型：
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作者：
LITTLEFIELD, JW;DUNN, DB
一些微生物、植物和哺乳动物的核糖核酸含有胸腺嘧啶、2-甲基腺嘌呤、6-甲基氨基嘌呤和6-二甲氨基嘌呤，其含量为尿嘧啶的0.05 ~ 3.7%。这些化合物主要以核苷形式分离得到，并与已知样品进行了色谱和光谱比较。每一种核糖体都被转化成相应的碱基。此外，分离出核糖体2和3磷酸；这一证据和其他证据表明，这4个碱基最初存在于核糖核酸的3[image]，5[image]-磷酸二酯连锁中。虽然在大多数微生物的核糖核酸中检测到所有4个甲基化碱基，但在肝脏核糖核酸中未发现胸腺嘧啶和2-甲基腺嘌呤，并且在2种植物病毒的核糖核酸中似乎没有这些碱基。
The ribonucleic acid from several microbial, plant and mammalian sources contained thymine, 2-methyladenine, 6-methylaminopurine and 6-dimethylamino-purine in amounts varying from 0.05 to 3.7% of the uracil. The compounds were isolated mainly as ribosides and identified by chromatographic and spectrosopic comparison with known samples. Each of the ribosides was converted into the corresponding base. In addition the riboside 2[image]-and 3[image]-phosphates were isolated; this and other evidence indicates that the 4 bases were originally present in 3[image],5[image]-phosphodiester linkage in the ribonucleic acid. Although all 4 methylated bases were detected in the ribonucleic acid from most of the microorganisms examined, thymine and 2-methyladenine were not found in liver ribonucleic acid and all these bases appeared to be absent from the ribonucleic acid of 2 plant viruses.