Cloning and characterization of two novel zebrafish P2X receptor subunits
Cloning and characterization of two novel zebrafish P2X receptor subunits
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DOI：
10.1016/s0006-291x(02)00760-x
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发表时间：
2002-07-26
影响因子：
3.1
通讯作者：
Voigt, MM
中科院分区：
文献类型：
--
作者：
Diaz-Hernandez, M;Cox, JA;Voigt, MM
在本报告中，我们描述了从斑马鱼 (Danio rerio) 克隆的两个 P2X 受体亚基的克隆和表征。一级序列分析表明，一个cDNA 编码哺乳动物P2X(4) 亚基的直向同源物，第二个cDNA 编码哺乳动物P2X(5) 亚基的直向同源物。 zP2X(4) 亚基形式；一种同源寡聚受体，对 ATP 表现出低亲和力 (EC50 = 274 +/- 48 muM)，对其他嘌呤能配体（如 AlphabetameATP、苏拉明和 PPADS）表现出非常低的亲和力 (EC50 > 500 muM)。正如哺乳动物直系同源物所见，zP2X(5) 亚基形成同源寡聚受体，产生非常小的全细胞电流。
In this report we describe the cloning and characterization of two P2X receptor subunits cloned from the zebrafish (Danio rerio). Primary sequence analysis suggests that one cDNA encodes an ortholog of the mammalian P2X(4) subunit and the second cDNA encodes the ortholog of the mammalian P2X(5) subunit. The zP2X(4) subunit form; a homo-oligomeric receptor that displays a low affinity for ATP (EC50 = 274 +/- 48 muM) and very low affinity (EC50 > 500 muM) for other purinergic ligands such as alphabetameATP, suramin, and PPADS. As seen with the mammalian orthologs, the zP2X(5) subunit forms a homo-oligomeric receptor that yields very small whole-cell currents (