Direct and instantaneous observation of intravenously injected substances using intravital confocal micro-videography
Direct and instantaneous observation of intravenously injected substances using intravital confocal micro-videography
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DOI：
10.1364/boe.1.001209
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发表时间：
2010-11-01
影响因子：
3.4
通讯作者：
Kataoka, Kazunori
中科院分区：
文献类型：
--
作者：
Matsumoto, Yu;Nomoto, Takahiro;Kataoka, Kazunori
我们描述的发展和应用活体共聚焦显微摄像可视化入口，分布和清除药物在各种组织和器官。我们使用尼康A1R共聚焦激光扫描显微镜系统连接到一个直立的ECLIPSE FN 1。尼康A1R允许同时进行四通道采集，速度为每秒30帧，同时保持512 x 512扫描点的高分辨率。我们的活体成像的关键技术是（1）向物镜透镜呈现平坦且垂直的表面，以及（2）在很少或没有出血的情况下暴露受试者，以便于光学进入多个组织和器官，以及（3）在不压迫血管的情况下将受试者与身体运动隔离，以及（4）插入尾静脉导管用于定时注射而不移动受试者。耳垂真皮组织无需手术即可触及。通过皮肤切口取出肝脏、肾脏和皮下肿瘤。为了对静脉内注射后化合物向组织中的初始外渗进行成像，在药物施用之前初始化电影采集。我们的技术可以作为一个强大的工具，调查生物机制和功能的静脉注射药物，具有空间和时间分辨率。(C)2010年美国光学学会
We describe the development and application of intravital confocal micro-videography to visualize entrance, distribution, and clearance of drugs within various tissues and organs. We use a Nikon A1R confocal laser scanning microscope system attached to an upright ECLIPSE FN1. The Nikon A1R allows simultaneous four channel acquisition and speed of 30 frames per second while maintaining high resolution of 512 x 512 scanned points. The key techniques of our intravital imaging are (1) to present a flat and perpendicular surface to the objective lens, and (2) to expose the subject with little or no bleeding to facilitate optical access to multiple tissues and organs, and (3) to isolate the subject from the body movement without compressing the blood vessels, and (4) to insert a tail vein catheter for timed injection without moving the subject. Ear lobe dermis tissue was accessible without surgery. Liver, kidney, and subcutaneous tumor were accessed following exteriorization through skin incision. In order to image initial extravasations of compounds into tissue following intravenous injection, movie acquisition was initialized prior to drug administration. Our technique can serve as a powerful tool for investigating biological mechanisms and functions of intravenously injected drugs, with both spatial and temporal resolution. (C) 2010 Optical Society of America