SUBUNIT SEPARATION (α, αprime;, β) OF CRYPTOMONAD BILIPROTEINS *
SUBUNIT SEPARATION (α, αprime;, β) OF CRYPTOMONAD BILIPROTEINS *
复制标题
隐单胞菌双脂蛋白的亚基分离 (α, αprime;, β) *
DOI：
10.1111/j.1751-1097.1986.tb05594.x
复制
发表时间：
1986
影响因子：
3.3
通讯作者：
R. Maccoll
中科院分区：
文献类型：
--
作者：
D. Guard;R. Maccoll
摘要-藻蓝蛋白645、藻蓝蛋白612和藻红蛋白545分别是从隐藻、绿球藻、绿半藻和红球藻透镜中分离的胆蛋白。该蛋白质具有α和β亚基。其在离子交换柱上通过使用尿素梯度和从溶剂中除去2-巯基乙醇而分离。这种分离确定α和β亚基不是通过二硫键连接的。此外，最近研究表明，巯基乙醇可在计算这些胆蛋白的发色团含量时产生虚假结果（Guard-Friar和MacColl，1984）。无巯基乙醇实验允许以快速和无伪影的方式分析发色团含量。当藻蓝蛋白645的离子交换层析通过改变尿素梯度的类型来操作时，获得两种不同的a亚基级分。这两个馏分具有相同的可见吸收光谱，但不同的氨基酸组成。因此，至少有两种不同的基因产物。负责A分队。藻蓝蛋白645的两个a亚基上唯一的发色团是酸性尿素中独特的697 nm胆色素。测定了它与巯基乙醇的反应性。藻红蛋白545的α亚基具有两种不同的胆素：隐小提琴和藻红胆素。藻蓝胆素是藻蓝蛋白612的α亚基上的发色团。
Abstract— Phycocyanin 645, phycocyanin 612 and phycoerythrin 545 are biliproteins isolated from the cryptomonad algae, Chroornonas species, Hemiselmis virescens and Rhodomonas lens, respectively. The protein has α and β subunits. which are separated on an ion exchange column by using a urea gradient and omitting 2‐mercaptoethanol from the solvent. This separation establishes that the α and β subunits are not joined by disulfide bonds. In addition it has recently been shown that mercaptoethanol can produce spurious results in the calculation of the chromophore contents of these biliproteins (Guard‐Friar and MacColl, 1984). The mercaptoethanol‐free experiments allow analysis of the chromophore content in a rapid and artifact‐free manner. When the ion exchange chromatography of phycocyanin 645 is manipulated by changing the type of urea gradient, two distinct a subunit fractions are obtained. These two fractions have identical visible absorption spectra but different amino acid compositions. At least two different gene products are, thus. responsible for the a subunits. The sole chromophore on the two a subunits of phycocyanin 645 is the unique 697‐nm bilin as seen in acidic urea. Its reactivity with mercaptoethanol is determined. The α subunits of phycoerythrin 545 have two different bilins: cryptoviolin and phycoerythrobilin. Phycocyanobilin is the chromophore on the α subunit of phycocyanin 612.