INCREASED NUMBERS OF CIRCULATING BASOPHIL PROGENITORS IN ATOPIC PATIENTS
INCREASED NUMBERS OF CIRCULATING BASOPHIL PROGENITORS IN ATOPIC PATIENTS
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DOI：
10.1016/0091-6749(85)90728-6
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发表时间：
1985-01-01
影响因子：
14.2
通讯作者：
BIENENSTOCK, J
中科院分区：
文献类型：
--
作者：
DENBURG, JA;TELIZYN, S;BIENENSTOCK, J
嗜碱性粒细胞募集到亲同型细胞抗体介导的超敏反应的位点在实验和临床情况中都有很好的记录。然而，组织嗜碱性粒细胞蓄积的潜在机制仍不清楚，可能涉及趋化性、细胞增殖或两者兼而有之。我们最近报道了在甲基纤维素中生长的粒细胞集落的组胺含量的基础上，在人类血液中循环的嗜碱性粒细胞/肥大细胞祖细胞的存在。在目前的研究中，我们分析了30例过敏症患者和25个可比较的对照组外周血嗜碱性粒细胞/肥大细胞祖细胞的频率通过分析单个粒细胞集落的组胺含量。在特应性患者的培养物中，40%的粒细胞集落含有组胺，而在对照受试者的培养物中，只有11%含有组胺（p < 0.001）。特应性受试者的粒细胞集落中每个集落的组胺含量以及每个集落中每个细胞的平均组胺含量较高，并且与集落大小或培养条件无关。粒细胞集落生长增强抗原刺激，外周血淋巴细胞条件培养基的特应性患者。组胺阳性集落在活动期比静止期更常见（p < 0.05）。这些结果是一致的假设，即嗜碱性粒细胞聚集在过敏反应的网站，至少部分通过招聘的祖细胞从循环和随后的分化原位淋巴因子。进一步研究使用造血测定可以阐明嗜碱性粒细胞的生产过敏性疾病的发展的贡献。
Recruitment of basophils to sites of homocytotropic antibody-mediated hypersensitivity reactions has been well documented in both experimental and clinical situations. Mechanisms underlying tissue basophil accumulation, however, remain unclear and may involve chemotaxis, cell proliferation, or both. We have recently reported the presence in human blood of circulating basophil/mast cell progenitors on the basis of histamine content of granulocyte colonies grown in methylcellulose. In the current studies we have analyzed the peripheral blood of 30 patients with atopy and 25 comparable control subjects for frequency of basophil/mast cell progenitors by analysis of the histamine content of individual granulocyte colonies. Forty percent of granulocyte colonies in cultures of atopic patients contained histamine in comparison to only 11% in cultures of control subjects (p < 0.001). Histamine content per colony as well as mean histamine per cell in each colony was higher in granulocyte colonies of atopic subjects and could not be related to colony size or culture conditions. Granulocyte colony growth was enhanced by antigen-stimulated, peripheral blood lymphomononuclear cell-conditioned media of atopic patients. Histamine-positive colonies were found more frequently in active versus quiescent atopic disease (p < 0.05). These results are consistent with the hypothesis that basophils accumulate at sites of allergic reactions at least in part by recruitment of progenitors from circulation and subsequent differentiation in situ in response to lymphokines. Further studies by use of hemopoietic assays could elucidate the contribution of basophil production to the development of allergic conditions.