Sorting Nexin 2 (SNX2): A Potential Marker of Active Thyrocytes in Normal and Hyperfunctioning Thyroid Disorders
Sorting Nexin 2 (SNX2): A Potential Marker of Active Thyrocytes in Normal and Hyperfunctioning Thyroid Disorders
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DOI：
10.1097/pai.0b013e31828badd3
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发表时间：
2014-04-01
影响因子：
1.6
通讯作者：
Itoh, Tomoo
中科院分区：
文献类型：
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作者：
Kanzawa, Maki;Hara, Shigeo;Itoh, Tomoo
分选连接蛋白（SNX）是一个大的，不同的一组细胞质和膜相关的蛋白质，在各种细胞过程中发挥作用，包括内吞作用，蛋白质运输，和跨膜蛋白的检索。在这项研究中，我们证明了SNX2在柱状和活跃的甲状腺滤泡细胞中表达，但在扁平的非活跃甲状腺细胞中不表达。形态计量学分析显示SNX2阳性和柱状细胞形态之间存在显著相关性。甲状腺组织连续切片的免疫组织化学染色表明，SNX2定位类似于分拣蛋白，一种由活跃的甲状腺细胞表达的蛋白质。SNX2在甲状腺细胞中的表达在大多数过度刺激的甲状腺疾病中特别显著和广泛，包括Graves病（73.3%的患者中弥漫性SNX2阳性）和功能性结节（93.8%的患者）。SNX2在过度刺激的滤泡上皮细胞中的免疫定位在SNXs家族成员中是特异性的。这些结果支持SNX2作为活性甲状腺细胞的新标记物的实用性，并反映了这些细胞中的内体运输活性。
Sorting nexins (SNXs) are a large, diverse group of cytoplasmic and membrane-associated proteins that function in a variety of cellular processes, including endocytosis, protein trafficking, and the retrieval of transmembrane proteins. In this study, we demonstrated that SNX2 is expressed in columnar and active thyroid follicular cells but not in flattened inactive thyrocytes. Morphometric analysis revealed a significant correlation between SNX2 positivity and columnar cell morphology. Immunohistochemical staining of serial sections of the thyroid tissue indicated that SNX2 localization was similar to sortilin, a protein expressed by active thyrocytes. Expression of SNX2 in thyrocytes is particularly marked and extensive in most hyperstimulated thyroid disorders, including Graves disease (diffusely SNX2 positive in 73.3% patients) and functioning nodules (93.8% patients). SNX2 immunolocalization in hyperstimulated follicular epithelial cells was specific among the SNXs family members examined. These results support the utility of SNX2 as a novel marker of active thyrocytes and reflect the endosomal trafficking activity in these cells.