Ageing is associated with reduced basal and stimulated release of nitric oxide by the coronary endothelium.
Ageing is associated with reduced basal and stimulated release of nitric oxide by the coronary endothelium.
复制标题
衰老与冠状动脉内皮基础一氧化氮的减少和刺激释放有关。
DOI：
10.1046/j.1365-201x.1996.451171000.x
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Magdi H. Yacoub
中科院分区：
文献类型：
--
作者：
Mohammed Amrani;A. T. Goodwin;C. Gray;Magdi H. Yacoub
众所周知，衰老过程与心脏的生化和功能变化有关。为了确定冠脉内皮细胞在静息状态和对药物刺激的反应中分泌一氧化氮(NO)的能力是否与年龄有关，我们研究了四组不同年龄的大鼠(分别为1、5、15和26个月)。根据冠脉流量对一氧化氮合酶抑制剂L-单甲基精氨酸的反应来评估内皮细胞基础释放的一氧化氮在较年轻的年龄组中较高。同样，在老年动物中，冠状动脉血流对5-羟色胺的反应减弱，5-羟色胺是一种选择性的内皮细胞分泌NO能力的探针。通过化学发光法直接测定冠脉流出液中的NO，证实了这一点。相比之下，对三硝酸甘油的反应似乎没有随着年龄的变化而改变。结论：在大鼠，冠脉内皮细胞基础和刺激释放的一氧化氮随年龄增加而恶化。
The ageing process is known to be associated with biochemical and functional changes in the heart. In an attempt to determine whether the ability of the coronary endothelium to secrete nitric oxide (NO) both at rest and in response to pharmacological stimulation is age dependent, we studied four groups of rats of different ages (1, 5, 15 and 26 months, respectively). Basal release of NO by endothelium as assessed by response of coronary flow to L-monomethylarginine, an inhibitor of NO synthase, was higher in the younger age groups. Similarly, the response of coronary flow to 5-hydroxytryptamine, a selective probe of endothelial capacity to secrete NO, was diminished in the older animals. This was confirmed by direct measurement of NO by chemiluminescence in the coronary effluent. In contrast, the response to glyceryl trinitrate appeared to be unaltered by age. It is concluded that in rats, basal and stimulated release of nitric oxide by the coronary endothelium deteriorates with age.