Imaging mass spectrometry of intact proteins from alcohol-preserved tissue specimens: Bypassing formalin fixation
Imaging mass spectrometry of intact proteins from alcohol-preserved tissue specimens: Bypassing formalin fixation
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DOI：
10.1021/pr800286z
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发表时间：
2008-08-01
影响因子：
4.4
通讯作者：
Caprioli, Richard M.
中科院分区：
文献类型：
--
作者：
Chaurand, Pierre;Latham, Joey C.;Caprioli, Richard M.
成像质谱正在成为一种关键技术，用于研究与潜在组织学直接相关的生物组织切片的分子含量。我们的大部分工作都是用新鲜冷冻的组织切片完成的，这些组织切片在组织活检取样和快速冷冻之间经历了最小的蛋白质降解，因此不需要向冷冻活检中添加防腐剂或固定剂。然而，在许多取样环境中，立即快速冷冻可能是不可能的，因此我们探索了使用乙醇保存的石蜡包埋组织标本进行蛋白质组学分析。仅溶剂保存的组织标本可在室温下长期保存，生成高质量的组织切片，并且蛋白质的化学变化很少（如果有）。使用小鼠器官，在组织脱水过程中所涉及的几个关键步骤进行了研究，以评估这种保存标本的分析和成像质谱研究的潜力。
Imaging mass spectrometry is becoming a key technology for the investigation of the molecular content of biological tissue sections in direct correlation with the underlying histology. Much of our work has been done with fresh-frozen tissue sections that has undergone minimal protein degradation between the time a tissue biopsy is sampled and the time it is snap-frozen so that no preserving or fixing agents need to be added to the frozen biopsy. However, in many sampling environments, immediate flash freezing may not be possible and so we have explored the use of ethanol-preserved, paraffin-embedded tissue specimens for proteomic analyses. Solvent-only preserved tissue specimens provide long-term preservation at room temperature, generation of high quality histological sections and little if any chemical alteration of the proteins. Using mouse organs, several key steps involved in the tissue dehydration process have been investigated to assess the potential of such preserved specimens for profiling and imaging mass spectrometry investigations.