BSA activated CdTe quantum dot nanosensor for antimony ion detection
BSA activated CdTe quantum dot nanosensor for antimony ion detection
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BSA 激活 CdTe 量子点纳米传感器，用于锑离子检测。
DOI：
10.1039/b915622d
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发表时间：
2010-01-01
期刊：
影响因子：
4.2
通讯作者：
Zhang, Shuangshuang
中科院分区：
文献类型：
--
作者：
Ge, Shenguang;Zhang, Congcong;Zhang, Shuangshuang
报道了一种基于巯基乙酸（TGA）修饰的CdTe量子点（QD）纳米粒子的新型纳米荧光传感器。这是第一个在受体-荧光团系统中使用QD纳米颗粒的锑离子传感器。采用水热法制备了水溶性的热重分析（TGA）修饰的CdTe量子点，并以NaHTe为Te前驱体合成了CdTe量子点。牛血清白蛋白（BSA）通过与TGA封端的CdTe的羧基相互作用的酰胺键与TGA封端的CdTe缀合。当锑离子进入BSA时，氮原子和氧原子的孤对电子参与配位，关闭QD发射并且导致荧光强度的急剧淬灭，从而允许检测低浓度的锑离子。利用该工作原理，基于量子点纳米粒子的锑离子传感器在0.10-22.0 μ g·L ~（-1）范围内具有良好的线性关系，检出限低于2.94 × 10 ~（-8）g·L ~（-1），相对标准偏差（RSD）为2.54%（n = 6）。在干扰研究中，锑敏感的TGA-QD-BSA传感器显示出良好的选择性。建立了一种简便、快速、灵敏、高选择性的锑的测定方法。方法用于真实的水样中锑的测定，结果满意。
A novel fluorescent nanosensor for Sb(3+) determination was reported based on thioglycolic acid (TGA)-capped CdTe quantum dot (QD) nanoparticles. It was the first antimony ion sensor using QD nanoparticles in a receptor-fluorophore system. The water-soluable TGA-capped CdTe QDs were prepared through a hydrothermal route, NaHTe was used as the Te precursor for CdTe QDs synthesis. Bovine serum albumin (BSA) conjugated to TGA-capped CdTe via an amide link interacting with carboxyl of the TGA-capped CdTe. When antimony ion enters the BSA, the lone pair electrons of the nitrogen and oxygen atom become involved in the coordination, switching off the QD emission and a dramatic quenching of the fluorescence intensity results, allowing the detection of low concentrations of antimony ions. Using the operating principle, the antimony ion sensor based on QD nanoparticles showed a very good linearity in the range 0.10-22.0 microg L(-1), with the detection limit lower than 2.94 x 10(-8) g L(-1) and the relative standard deviation (RSD) 2.54% (n = 6). In a study of interferences, the antimony-sensitive TGA-QD-BSA sensor showed good selectivity. Therefore, a simple, fast, sensitive, and highly selective assay for antimony has been built. The presented method has been applied successfully to the determination of antimony in real water samples (n = 6) with satisfactory results.