Increased expression of mitochondrial glycerophosphate dehydrogenase and antioxidant enzymes in prostate cancer cell lines/cancer
Increased expression of mitochondrial glycerophosphate dehydrogenase and antioxidant enzymes in prostate cancer cell lines/cancer
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DOI：
10.1080/10715760701579314
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发表时间：
2007-01-01
影响因子：
3.3
通讯作者：
Singh, Gurmit
中科院分区：
文献类型：
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作者：
Chowdhury, Subir K. Roy;Raha, Sandeep;Singh, Gurmit
线粒体甘油磷酸脱氢酶（mGPDH）参与前列腺癌细胞系（LNCaP、DU 145、PC 3和CL 1）中ROS的产生。目前的研究表明，前列腺癌细胞中mGPDH的mRNA水平比正常前列腺上皮细胞系PNT 1A高3.3-8.9倍。这与mGPDH的酶活性和蛋白水平一致。然而，细胞色素c氧化酶（考克斯）活性在雄激素非依赖性前列腺癌细胞系中下调2.9-3.2倍。抗氧化酶、过氧化氢酶、MnSOD和CuZnSOD的水平在前列腺癌细胞系中上调。此外，观察到与来自对照小鼠的肝组织相比，来自所有患有癌症的小鼠的肝组织中mGPDH的活性显著更高。这些数据表明，由于高度糖酵解的环境，mGPDH的上调有助于ROS产生的总体增加，并可能导致癌症的进展。
The involvement of mitochondrial glycerophosphate dehydrogenase (mGPDH) has previously been established in the production of ROS in prostate cancer cell lines (LNCaP, DU145, PC3 and CL1). The current study demonstrates that the mRNA level of mGPDH in prostate cancer cells is 3.3-8.9-fold higher compared to the normal prostate epithelial cell line, PNT1A. This is consistent with the enzymatic activity and protein level of mGPDH. However, cytochrome c oxidase (COX) activity is 2.9-3.2-fold down-regulated in androgen- independent prostate cancer cell lines. The level of antioxidant enzymes, catalase, MnSOD and CuZnSOD are up-regulated in prostate cancer cell lines. Furthermore, it was observed that the activity of mGPDH is significantly higher in liver tissues from all mice with cancer compared to liver tissues from control mice. These data suggest that the up- regulation of mGPDH, due to a highly glycolytic environment, contributes to the overall increase in ROS generation and may result in the progression of the cancer.