Induction ofhemocyte apoptosis byOvomermis sinensis: Implications for host immune suppression
Induction ofhemocyte apoptosis byOvomermis sinensis: Implications for host immune suppression
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中华卵丝菌诱导血细胞凋亡：对宿主免疫抑制的影响
DOI：
10.1016/j.jip.2018.10.011
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发表时间：
2018
影响因子：
3.4
通讯作者：
Guoxiu Wang
中科院分区：
文献类型：
--
作者：
Zhenlong Jiao;Guigui Wena;Guoxiu Wang
昆虫病原线虫是鳞翅目一些常见害虫的寄生虫。中华夜蛾能够克服宿主的免疫系统，并最终在其出现时杀死宿主。我们提供了深入了解如何吸血线虫克服宿主棉铃虫的免疫反应。我们的研究结果表明，中华O. O. ensis可以通过血细胞结瘤、扩散行为、溶菌酶活性和黑色素化来抑制宿主的免疫反应。然而，中华O. O. ensis并未通过免疫基因激活来抑制宿主的免疫应答。此外，对中华绒螯蟹免疫抑制策略的研究表明，中华绒螯蟹不抑制宿主效应分子，但会减少血细胞数量。流式细胞术分析显示，宿主血细胞在24h内凋亡，72h后无血细胞存在。此外，我们的体内和体外研究表明，当血细胞增殖受到抑制时，中华o.s sinensis的存活率会增加。我们的研究结果表明，中华绒螯蟹通过诱导宿主血细胞凋亡来抑制宿主的免疫反应。
The entomopathogenic nematode, Ovomermis sinensis, is a parasite of some common lepidopteran pests. O. sinensis is able to overcome the immune system of its hosts and eventually kill the hosts when it emerges. We provide insight into how the mermithid nematode overcomes the immune response of the host Helicoverpa armigera. Our results indicate that O. sinensis actively inhibits host immune responses as evidenced by hemocyte nodulation, spreading behavior, lysozyme activity and melanization. However, O. sinensis did not inhibit host immuneresponsesthroughimmunegeneactivation.Moreover,theresearchontheimmunedepressivestrategies of O. sinensis revealed that the parasite did not inhibit host effector molecules, but did reduce the number of hemocytes. Flow cytometry analysis revealed that the host hemocytes were apoptotic within 24h, and no hemocytes were present after 72h. In addition, our in vivo and in vitro studies showed that the survival rate of O. sinensis was increased when hemocyte proliferation was inhibited. Our findings suggest that O. sinensis inhibited host immune responses by inducing apoptosis of host hemocytes..