ROS of Distinct Sources and Salicylic Acid Separate Elevated CO2-Mediated Stomatal Movements in Arabidopsis
ROS of Distinct Sources and Salicylic Acid Separate Elevated CO2-Mediated Stomatal Movements in Arabidopsis
复制标题
不同来源的活性氧和水杨酸分离拟南芥中二氧化碳介导的气孔运动升高
DOI：
10.3389/fpls.2020.00542
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发表时间：
2020-05-08
影响因子：
5.6
通讯作者：
Liang, Yun-Kuan
中科院分区：
文献类型：
--
作者：
He, Jingjing;Zhang, Ruo-Xi;Liang, Yun-Kuan
升高的CO2（eCO（2））通常会降低叶片气孔开度和密度，从而影响植物生理和生产力。我们以前已经证明，拟南芥BIG蛋白区分eCO（2）诱导的气孔关闭和eCO（2）抑制的气孔打开的过程。然而，这种作用的机械基础还没有完全理解。在这里，我们表明，在大突变体中，eCO（2）诱导的活性氧（ROS）的产生在气孔关闭诱导中受到损害，但在气孔开放抑制中没有。药理学和遗传学研究表明，NADPH氧化酶和细胞壁过氧化物酶产生的ROS有助于eCO（2）诱导的气孔关闭，而eCO（2）抑制光诱导的气孔开放可能依赖于NADPH氧化酶而不是细胞壁过氧化物酶产生的ROS。与JA和阿坝一样，SA是eCO（2）诱导的ROS产生和气孔关闭所必需的。相反，这三种信号在eCO（2）抑制的气孔开放中没有显著作用，揭示了植物激素信号通路在诱导气孔关闭和eCO（2）抑制气孔开放中的不同作用。总之，这项研究将SA添加到植物激素列表中，与来自不同来源的ROS一起区分eCO（2）介导的气孔运动的两个分支。
Elevated CO2 (eCO(2)) often reduces leaf stomatal aperture and density thus impacts plant physiology and productivity. We have previously demonstrated that the Arabidopsis BIG protein distinguishes between the processes of eCO(2)-induced stomatal closure and eCO(2)-inhibited stomatal opening. However, the mechanistic basis of this action is not fully understood. Here we show that eCO(2)-elicited reactive oxygen species (ROS) production in big mutants was compromised in stomatal closure induction but not in stomatal opening inhibition. Pharmacological and genetic studies show that ROS generated by both NADPH oxidases and cell wall peroxidases contribute to eCO(2)-induced stomatal closure, whereas inhibition of light-induced stomatal opening by eCO(2) may rely on the ROS derived from NADPH oxidases but not from cell wall peroxidases. As with JA and ABA, SA is required for eCO(2)-induced ROS generation and stomatal closure. In contrast, none of these three signals has a significant role in eCO(2)-inhibited stomatal opening, unveiling the distinct roles of plant hormonal signaling pathways in the induction of stomatal closure and the inhibition of stomatal opening by eCO(2). In conclusion, this study adds SA to a list of plant hormones that together with ROS from distinct sources distinguish two branches of eCO(2)-mediated stomatal movements.