DIFFERENTIAL EXPRESSION OF GAP JUNCTION CONNEXINS IN ENDOCRINE AND EXOCRINE GLANDS
DIFFERENTIAL EXPRESSION OF GAP JUNCTION CONNEXINS IN ENDOCRINE AND EXOCRINE GLANDS
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DOI：
10.1210/en.133.5.2371
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发表时间：
1993-11-01
期刊：
影响因子：
4.8
通讯作者：
ORCI, L
中科院分区：
文献类型：
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作者：
MEDA, P;PEPPER, MS;ORCI, L
我们已经研究了三个间隙连接蛋白及其相应的mRNAS的各种内分泌和外分泌大鼠腺体的分泌细胞的表达。通过免疫染色冷冻切片（间接免疫荧光）与连接蛋白（Cx）26，32和43的抗体，并通过杂交总腺RNA（北方印迹）与这些蛋白质的cRNA，我们已经发现，几个内分泌腺，（垂体、甲状旁腺、胰岛和肾上腺）表达Cx43，Cx26表达水平不同，不表达Cx32，而一些外分泌腺表达Cx43，（泪腺、唾液腺、胰腺、前列腺和精囊）表达高水平的Cx32和可变水平的Cx26，但不表达Cx43。因此，不同的蛋白质组构成了内分泌腺和外分泌腺的pp连接。与此同时，发现内分泌腺（甲状腺）在将分泌产物释放到血管区室之前先将其分泌到细胞外，表达Cx43和Cx32，而外分泌腺（甲状腺）表达Cx43和Cx32。具有信息素作用的包皮腺表达Cx43，这些观察结果表明，缝隙连接连接蛋白的差异表达可能需要指定分泌型细胞的内分泌或外分泌分化。cell.
We have investigated the expression of three gap junction proteins and their corresponding mRNAS by secretory cells of a variety of endocrine and exocrine rat glands. By immunostaining cryostat sections (indirect immunofluorescence) with antibodies against connexins (Cx) 26, 32, and 43 and by hybridizing total glandular RNA (Northern blot) with cRNAs for these proteins, we have found that several endocrine glands (pituitary, parathyroid, pancreatic islets, and adrenal) express Cx43, variable levels of Cx26, and no Cx32, whereas several exocrine glands (lacrimal gland, salivary glands, pancreas, prostate, and seminal vesicle) express high levels of Cx32 and variable levels of Cx26, but no Cx43. Thus, different sets of proteins comprise the pp junctions of endocrine and exocrine glands. Together with the findings that an endocrine gland (thyroid) that discharges secretory products extracellularly before releasing them in the vascular compartment expresses both Cx43 and Cx32 and that an exocrine gland (preputial gland) that has a pheromonal role expresses Cx43, these observations suggest that the differential expression of gap junction connexins may be required to specify the endocrine or exocrine differentiation of a secretory cell.