Correlation of cell surface antigen expression on human thymocytes by multi-color flow cytometric analysis: implications for differentiation.
Correlation of cell surface antigen expression on human thymocytes by multi-color flow cytometric analysis: implications for differentiation.
复制标题
通过多色流式细胞术分析人胸腺细胞表面抗原表达的相关性：对分化的影响。
作者：
Lewis L. Lanier;James P. Allison;J. Phillips
胸腺细胞在胸腺成熟过程中经历一系列复杂的表型和基因型变化。这种动态过程涉及某些细胞表面分化抗原表达的定性和定量变化。本研究应用多色免疫荧光技术和多参数流式细胞术直接检测了正常人胸腺细胞T细胞分化抗原表达的关系。这些研究的结果为胸腺成熟过程中抗原表达的复杂性提供了新的见解，并表明CD 3/T细胞抗原受体复合物在胸腺细胞发育的早期表达。通过使用多参数流式细胞术分析对抗原表达的直接定量测量也表明某些抗原的定量共调节（例如，CD 3和CD 5）。
Thymocytes undergo a complex series of phenotypic and genotypic changes during maturation in the thymus. This dynamic process involves qualitative and quantitative changes in the expression of certain cell surface differentiation antigens. In this study, we have directly examined the relationship of T cell differentiation antigen expression on normal human thymocytes by using multi-color immunofluorescence and multi-parameter flow cytometric analysis. The results from these studies have provided new insights into the complexity of antigen expression during thymic maturation and suggest that the CD3/T cell antigen receptor complex is expressed early in the development of thymocytes. Direct quantitative measurements of antigen expression by using multi-parameter flow cytometric analysis also suggest quantitative co-regulation of certain antigens (e.g., CD3 and CD5) during thymic maturation.