Thoracoscopic cell sheet transplantation with a novel device
Thoracoscopic cell sheet transplantation with a novel device
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DOI：
10.1002/term.161
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发表时间：
2009-06-01
影响因子：
3.3
通讯作者：
Okano, Teruo
中科院分区：
文献类型：
--
作者：
Maeda, Masanori;Yamato, Masayuki;Okano, Teruo
在温度响应性培养表面制备可移植细胞片的再生医学已在临床应用中取得成功，包括皮肤和角膜治疗。此前，我们报道了与传统材料相比，将成纤维细胞片移植到受伤的肺部在封堵术中气漏方面具有很大优势。在此，我们报道了一种在内镜手术（如电视辅助胸腔镜手术（VATS））中用于细胞片微创移植的新型装置。该新型装置是通过计算机辅助设计（CAD）系统设计的，其三维（3D）数据被传输到3D打印机。利用这种快速成型系统，使用一种经批准可用于临床的市售光聚合物制造了细胞片移植装置。将正方形细胞片（24×24毫米）成功移植到置于人体模型中的猪肺伤口部位，该装置通过一个12毫米的端口插入。这种装置将能够使细胞片以更小的侵入性移植到多种内脏器官上。版权所有（c）2009约翰威立父子有限公司
Regenerative medicine with transplantable cell sheets fabricated on temperature-responsive culture surfaces has been successfully achieved in clinical applications, including skin and cornea treatment. Previously, we reported that transplantation of fibroblast cell sheets to wounded lung had big advantages for sealing intraoperative air leaks compared with conventional materials. Here, we report a novel device for minimally invasive transplantation of cell sheets in endoscopic surgery, such as video-assisted thoracoscopic surgery (VATS). The novel device was designed with a Computer-aided design (CAD) system, and the three-dimensional (3D) data were transferred to a 3D printer. With this rapid prototyping system, the cell sheet transplantation device was fabricated using a commercially available photopolymer approved for clinical use. Square cell sheets (24 x 24 mm) were successfully transplanted onto wound sites of porcine lung placed in a human body model, with the device inserted through a 12 mm port. Such a device would enable less invasive transplantation of cell sheets onto a wide variety of internal organs. Copyright (c) 2009 John Wiley & Sons, Ltd.