RAPID IDENTIFICATION OF A POINT MUTATION OF THE MYCOBACTERIUM-TUBERCULOSIS CATALASE-PEROXIDASE (KATG) GENE ASSOCIATED WITH ISONIAZID RESISTANCE
RAPID IDENTIFICATION OF A POINT MUTATION OF THE MYCOBACTERIUM-TUBERCULOSIS CATALASE-PEROXIDASE (KATG) GENE ASSOCIATED WITH ISONIAZID RESISTANCE
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DOI：
10.1093/infdis/171.1.240
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发表时间：
1995-01-01
影响因子：
6.4
通讯作者：
KLINE, BC
中科院分区：
文献类型：
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作者：
COCKERILL, FR;UHL, JR;KLINE, BC
测定了15株对异烟肼敏感的结核分枝杆菌的过氧化氢酶-过氧化物酶（katG）基因全序列。异烟肼MIC大于或等于1.0 μ g/mL的9个菌株中有5个菌株有一个或多个错义突变，所有5个菌株在密码子463处都有一个共同的G -> T颠换，导致精氨酸被亮氨酸取代，并丢失NciI或MspI限制性位点。6株过氧化氢酶-过氧化物酶基因第463位密码子中没有一个含有异烟肼MIC亮氨酸的菌株在M.异烟肼MIC大于或等于1.0 μ g/mL的结核菌株。
The complete catalase-peroxidase (katG) gene DNA sequence was determined for 15 strains of Mycobacterium tuberculosis with a wide range of susceptibility to isoniazid. Five of 9 strains with isoniazid MICs greater than or equal to 1.0 mu g/mL had one or more missense mutations and all 5 strains had a common G --> T transversion in codon 463, causing the replacement of arginine with leucine and the loss of an NciI or MspI restriction site. None of 6 strains with an isoniazid MIC leucine in codon 463 of the catalase-peroxidase gene occurs in a significant fraction (44.2%) of M. tuberculosis strains with isoniazid MICs greater than or equal to 1.0 mu g/mL.